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MCF7 nuclear extract lysate (H2O2 post treated) (ab14863)

Submit a review Q&A (3)
Functional Studies - MCF7 nuclear extract lysate (H<sub>2</sub>O<sub>2</sub> post treated) (ab14863)

    Overview

    • Product name

      MCF7 nuclear extract lysate (H2O2 post treated)
      See all MCF7 lysates

    • General notes

      Post-collection treatment consists in exposing the nuclear extract to a final concentration of 200 mM H2O2 for 20 min on ice. The protein content has been determined by a Bradford-based assay.

    Properties

    • Mycoplasma free

      Yes

    • Form

      Liquid

    • Storage instructions

      Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

    • Storage buffer

      pH: 7.50
      Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
    • Concentration information loading...

    • Research areas

    • Background

      MCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.

    Images

    • Functional Studies - MCF7 nuclear extract lysate (H<sub>2</sub>O<sub>2</sub> post treated) (ab14863)
      Functional Studies - MCF7 nuclear extract lysate (H2O2 post treated) (ab14863)

      Each lot has been tested for Sp1 activity by using the TransAM™ Sp1 Kit. The signal intensity for Sp1 activity in each lot is compared to the signal intensity obtained with H2O2-post treated extracts from MCF-7 cells. Once the signals are blanked, the ratio between the signals from untreated extracts over treated extracts used at 5 µg/well must be above 4.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab14863? Please let us know so that we can cite the reference in this datasheet.

    ab14863 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    Phoned looking for a control lysate for BCL9 antibody.

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    Answer

    Thank you for contacting us.

    We do not know what effect H2O2 stress will have on expression of BCL9 in MCF7 cells therefore I would recommend perhaps running H2O2 treated MCF7 lysate alongside untreated if possible to determine affect.

    We do have other tissue types that can be used as control signal. OurHeLa Nuclear Lysate, (ab14655:https://www.abcam.com/HeLa-Human-epithelial-carcinoma-cell-line-Nuclear-Lysate-ab14655.html) gave the next best signal after MCF7 with ab37305. This product is in stock with next day delivery.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    what exactly does MCF-7 stand for?

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    Answer

    Thank you for your enquiry. MCF stands for Michigan Cancer Foundation, the Detroit institution that derived this cell line. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Question

    Was this lysate diluted to achieve the final concentration of 2.5mg/ml? How much was it diluted? Does this extract contain DNA?

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    Answer

    Thank you for your enquiry. The MCF-7 nuclear extracts have been collected in Lysis Buffer. The Lysis Buffer consists of 20 mM Hepes pH 7.5, 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM ß-glycerophosphate, 1 mM DTT and protease inhibitor. If the extract was diluted to reach the concentration of 2.5 mg/ml (actual concentration), it is in this lysis buffer. I don't think that if it was diluted it was more than 2 to 3 fold, but I don't have the exact info. The lysate does not contain DNA. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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