Mouse brain tissue lysate - mitochondrial extract (contains SDS/DTT) (ab110345)

As we discussed over the phone, I have contacted the lab at Mitosciences to get their input into this query. The mouse brain mitochondrial lysate has been prepared using standard mitochondrial isolation procedures. These procedures separate the mitochondria based on differential centrifugation - mitochondria pellet at 12000g after having removed larger cellular components. As such they are highly enriched for mitochondria but not pure. The quality control of the lysate has been performed using mitochondrial antibody cocktails, please see the image attached. This is true of the kit ab110168 as well.

To make an assessment of purity one would need to look at a variety of non mitochondrial components to determine the level of contamination. So in short if you are looking for guaranteed highly pure mitochondria (for example >90%) we wouldn’t recommend these products.

Essentially very pure mitochondiral fractions are best prepared by taking crude mitos and spinning over a gradient (traditionally percoll, metrizamide sometimes used too). This is not a very scalable procedure so not practical for us to do.