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  1. Link

    products/tissue-lysates/mouse-liver-tissue-lysate-total-protein-ab29301.pdf

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Kits/ Lysates/ Other Lysates Whole Cell Lysates Mouse Gastrointestinal
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Mouse liver tissue lysate - total protein (ab29301)

  • Datasheet
  • SDS
Submit a review Q&A (1)

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Overview

  • Product name

    Mouse liver tissue lysate - total protein
    See all Liver lysates
  • General notes


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Mycoplasma free

    Yes
  • Form

    Liquid
  • Storage instructions

    Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage buffer

    Constituents: 1% 4-Nonylphenol, branched, ethoxylated, Potassium chloride, 0.1% Bromophenol blue, Magnesium chloride, 1.2% Beta mercaptoethanol, 2% Sodium lauryl sulfate, HEPES, Sucrose, 10% Glycerol

    With Protease inhibitor cocktail/EDTA/Sodium deoxycholate
  • Concentration information loading...
  • Lysate notes

    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
  • Research areas

    • Kits/ Lysates/ Other
    • Lysates
    • Whole Cell Lysates
    • Mouse
    • Gastrointestinal
    • Kits/ Lysates/ Other
    • Lysates
    • Whole Cell Lysates
    • Mouse
    • Other

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab29301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.
Notes
WB
Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab29301? Please let us know so that we can cite the reference in this datasheet.

ab29301 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Inquiry: Hi, I have some anti-scavenger BI + BII antibody (ab36970) and I want to prepare a positive control to use with it. On the data sheet total mouse liver lysate was used. Could you tell me how this liver lysate was prepared? Thanks

Read More

Abcam community

Verified customer

Asked on Jul 11 2012

Answer

Thank you for your enquiry

We obtain this product from an external source and unfortunately they are not able to provide specific information about the sample preparation.

I reviewed the literature and found the following protocol that you may find useful:

1. Used 1-2 liver lobes.
2. Homogenize tissue in PBS/Protease Inhibitor cocktail.
3. After centrifugation resuspend the pellet in approx. 400µl RIPA lysis buffer 30min (vortex every 5-10 min).
4. Centrifuge to give whole cell extracts.
5. To improve the amount of sample extracted, repeat this lysis step with the pellet from the centrifugation and pool the supernatant of the 1st and the 2nd lysis.

We also stock a range of liver lysates:

Liver (Mouse) Whole Cell Lysate - normal tissue, 0 days old
www.abcam.com/ab7264

Liver (Mouse) Whole Cell Lysate - normal tissue, 7 days old
www.abcam.com/ab7266

Liver (Mouse) Whole Cell Lysate - normal tissue
www.abcam.com/ab29301

Liver (Human) Tissue Lysate - adult normal tissue
www.abcam.com/ab29889

Please let me know if there is any more information you need.

Read More

Abcam Scientific Support

Answered on Jul 11 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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