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  1. Link

    products/tissue-lysates/mouse-skeletal-muscle-tissue-lysate-total-protein-ab29711.pdf

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Kits/ Lysates/ Other Lysates Whole Cell Lysates Mouse Muscle/Skin/Skeleton
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Mouse skeletal muscle tissue lysate - total protein (ab29711)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (2)

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Overview

  • Product name

    Mouse skeletal muscle tissue lysate - total protein
  • General notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Mycoplasma free

    Yes
  • Form

    Liquid
  • Storage instructions

    Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage buffer

    pH: 7.90
    Constituents: 5% 4-Nonylphenol, branched, ethoxylated, 0.1% Potassium chloride, 0.1% Magnesium chloride, 0.1% HEPES, 0.1% Sucrose, 10% Glycerol, 0.1% EDTA, 0.1% Sodium deoxycholate

    With cocktail of Protease inhibitor
  • Concentration information loading...
  • Lysate notes

    Protein is isolated from mouse (Balb/C) whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

  • Research areas

    • Kits/ Lysates/ Other
    • Lysates
    • Whole Cell Lysates
    • Mouse
    • Muscle/Skin/Skeleton

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab29711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.
Notes
WB
Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab29711? Please let us know so that we can cite the reference in this datasheet.

ab29711 has been referenced in 2 publications.

  • Krekeler BN  et al. Effects of Tongue Exercise Frequency on Tongue Muscle Biology and Swallowing Physiology in a Rat Model. Dysphagia 35:918-934 (2020). PubMed: 32130514
  • Kletzien H  et al. Myogenic marker expression as a function of age and exercise-based therapy in the tongue. Exp Gerontol 142:111104 (2020). PubMed: 33017670

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

I have some questions concerning the following items:
- human (ab29330), mouse (ab29711) and rat (ab29376) skeletal muscle extracts

- would it be possible to have more precisions concerning the donors, especially the exact nature of skeletal muscle for mouse, rat and human?

- I generally use ACTB as loading control. Is normal that I do not detect and ACTB in muscles?

Read More

Abcam community

Verified customer

Asked on May 20 2013

Answer


I have heard back from the lab concerning donor information for the lots requested of human, mouse and rat skeletal tissue lysates.


Skeletal Muscle (Human) Tissue Lysate: 24 year old normal male

Skeletal Muscle (Rat) Tissue Lysate: 44 week old normal rat

Skeletal Muscle (Mouse) Tissue Lysate: 8 week old normal mouse


As for the use of ACTB as a loading control, it is normal that no or low levels are detected in muscles. This is because Beta Actin is a cytoskeletal actin as opposed to a muscle actin. Six actin isoforms are known: two sarcomeric (a-skeletal and a-cardiac), two smooth muscle actins (a and g), and two non-muscle, cytoskeletal actins (b and g). We would suggest using GAPDH as a loading control instead.

Read More

Abcam Scientific Support

Answered on May 20 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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