Mouse skeletal muscle tissue lysate - total protein (ab29711)
Overview
-
Product name
Mouse skeletal muscle tissue lysate - total protein -
General notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: WBmore details
Properties
-
Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting. -
Storage buffer
pH: 7.90
Constituents: 5% 4-Nonylphenol, branched, ethoxylated, 0.1% Potassium chloride, 0.1% Magnesium chloride, 0.1% HEPES, 0.1% Sucrose, 10% Glycerol, 0.1% EDTA, 0.1% Sodium deoxycholate
With cocktail of Protease inhibitor -
Concentration information loading... -
Lysate notes
Protein is isolated from mouse (Balb/C) whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
-
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab29711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.
|
| Notes |
|---|
|
WB
Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab29711 has been referenced in 2 publications.
- Krekeler BN et al. Effects of Tongue Exercise Frequency on Tongue Muscle Biology and Swallowing Physiology in a Rat Model. Dysphagia 35:918-934 (2020). PubMed: 32130514
- Kletzien H et al. Myogenic marker expression as a function of age and exercise-based therapy in the tongue. Exp Gerontol 142:111104 (2020). PubMed: 33017670