Mouse spleen tissue lysate - total protein (ab7937)
Overview
-
Product name
Mouse spleen tissue lysate - total protein
See all Spleen lysates -
General notes
Source: White laboratory miceMouse spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris were removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: WBmore details
Properties
-
Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituents: 60.05% Water, 12.5% Glycerol (glycerin, glycerine), 9% Tris HCl, 7.7% DTT, 4.4% Sodium chloride, 1% Triton-X-100, 1% Sodium deoxycholate, 1.1% Sodium lauryl sulfate, 0.15% EDTA disodium salt, 0.5% Aprotinin, 0.5% Leupeptin hemisulfate, 0.09% PMSF, 0.01% Bromophenol blue -
Concentration information loading... -
Lysate notes
Mouse spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris were removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7937 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Mouse spleen tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Mouse spleen tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab7937 has been referenced in 3 publications.
- Liu H et al. Cryptotanshinone Protects against PCOS-Induced Damage of Ovarian Tissue via Regulating Oxidative Stress, Mitochondrial Membrane Potential, Inflammation, and Apoptosis via Regulating Ferroptosis. Oxid Med Cell Longev 2022:8011850 (2022). PubMed: 35419170
- Wen X et al. CircRNA-016901 silencing attenuates irradiation-induced injury in bone mesenchymal stem cells via regulating the miR-1249-5p/HIPK2 axis. Exp Ther Med 21:355 (2021). PubMed: 33732328
- Yang Y et al. Cryptotanshinone alleviates polycystic ovary syndrome in rats by regulating the HMGB1/TLR4/NF-?B signaling pathway. Mol Med Rep 22:3851-3861 (2020). PubMed: 32901834