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    products/tissue-lysates/rat-heart-tissue-lysate-mitochondrial-extract-ab110347.pdf

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Rat heart tissue lysate - mitochondrial extract (ab110347)

  • Datasheet
  • SDS
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Overview

  • Product name

    Rat heart tissue lysate - mitochondrial extract
    See all Rat Heart lysates
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

    2 mg of rat heart mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE. Immunocapture, however could also be used for electrophoresis and Western blotting, and Enzyme Isolations. 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

  • Tested applications

    Suitable for: IP, SDS-PAGE, WBmore details

Properties

  • Mycoplasma free

    Yes
  • Form

    Liquid
  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.5
    Constituents: 0.02% PMSF, 0.16% Tris, 0.89% EDTA, 8.56% Sucrose
  • Concentration information loading...
  • Lysate notes

    2 mg of rat heart mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE. Immunocapture, however could also be used for electrophoresis and Western blotting, and Enzyme Isolations. 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
SDS-PAGE
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
Notes
IP
Use at an assay dependent concentration.
SDS-PAGE
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

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References (0)

Publishing research using ab110347? Please let us know so that we can cite the reference in this datasheet.

ab110347 has not yet been referenced specifically in any publications.

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