Rat heart tissue lysate - mitochondrial extract (ab110347)
Overview
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Product name
Rat heart tissue lysate - mitochondrial extract
See all Rat Heart lysates -
General notes
Product was previously marketed under the MitoSciences sub-brand.
2 mg of rat heart mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE. Immunocapture, however could also be used for electrophoresis and Western blotting, and Enzyme Isolations. 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.
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Tested applications
Suitable for: IP, SDS-PAGE, WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.5
Constituents: 0.02% PMSF, 0.16% Tris, 0.89% EDTA, 8.56% Sucrose -
Concentration information loading... -
Lysate notes
2 mg of rat heart mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE. Immunocapture, however could also be used for electrophoresis and Western blotting, and Enzyme Isolations. 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| SDS-PAGE |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration.
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| Notes |
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IP
Use at an assay dependent concentration. |
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SDS-PAGE
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab110347 has not yet been referenced specifically in any publications.