Rat heart tissue lysate - mitochondrial extract (contains SDS/DTT) (ab110341)
Overview
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Product name
Rat heart tissue lysate - mitochondrial extract (contains SDS/DTT)
See all Rat Heart lysates -
General notes
Product was previously marketed under the MitoSciences sub-brand.
50 µg of rat heart mitochondria solubilized in SDS-PAGE loading buffer and supplemented with reducing agent. This sample is ready for SDS-electrophoresis and acts as a positive control in Western blotting applications.
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Tested applications
Suitable for: SDS-PAGE, WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.5
Constituent: 99% SDS-PAGE Sample Buffer -
Concentration information loading... -
Lysate notes
50 µg of rat heart mitochondria solubilized in SDS-PAGE loading buffer and supplemented with reducing agent. This sample is ready for SDS-electrophoresis and acts as a positive control in Western blotting applications.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| SDS-PAGE |
Use at an assay dependent dilution.
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| WB |
Use at an assay dependent dilution. 10.0 µg/lane
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| Notes |
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SDS-PAGE
Use at an assay dependent dilution. |
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WB
Use at an assay dependent dilution. 10.0 µg/lane |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab110341 has been referenced in 2 publications.
- Belosludtsev KN et al. Alisporivir Treatment Alleviates Mitochondrial Dysfunction in the Skeletal Muscles of C57BL/6NCrl Mice with High-Fat Diet/Streptozotocin-Induced Diabetes Mellitus. Int J Mol Sci 22:N/A (2021). PubMed: 34502433
- Frapin M et al. Maternal Protein Restriction in Rats Alters the Expression of Genes Involved in Mitochondrial Metabolism and Epitranscriptomics in Fetal Hypothalamus. Nutrients 12:N/A (2020). PubMed: 32438566