Product nameSaos-2 nuclear extract lysate
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 7.90
Constituents: 0.00021% Sodium molybdate, 0.216% Beta glycerophosphate, 0.018% Sodium orthovanadate, 0.422% Sodium fluoride, 0.015% DTT, 0.48% HEPES, 0.003% EDTA, 20% Glycerol, 2.34% Sodium chloride
With Protease inhibitors
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BackgroundThe Saos-2 cells possess several osteoblastic features and could be useful as a permanent line of human osteoblast-like cells and as a source of bone-related molecules. They may express high levels of epidermal growth factor (EGF) and transforming growth factor beta (type 1 and type 2).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab14891 has not yet been referenced specifically in any publications.