Recombinant Anti-CD42b antibody [EPR6995] (ab134087)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6995] to CD42b
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD42b antibody [EPR6995]
See all CD42b primary antibodies -
Description
Rabbit monoclonal [EPR6995] to CD42b -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD42b aa 1-100. The exact sequence is proprietary.
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Positive control
- Human platelet lysate, Human spleen tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.70 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6995 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 143 kDa (predicted molecular weight: 69 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 143 kDa (predicted molecular weight: 69 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. -
Involvement in disease
Non-arteritic anterior ischemic optic neuropathy
Bernard-Soulier syndrome
Bernard-Soulier syndrome A2, autosomal dominant
Pseudo-von Willebrand disease -
Sequence similarities
Contains 7 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
Post-translational
modificationsGlycocalicin, which is approximately coextensive with the extracellular part of the molecule, is cleaved off by calpain during platelet lysis. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2811 Human
- Omim: 606672 Human
- SwissProt: P07359 Human
- Unigene: 1472 Human
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Alternative names
- Antigen CD42b alpha antibody
- Antigen CD42b-alpha antibody
- BDPLT1 antibody
see all
Images
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Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling CD42b with ab134087 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Anti-CD42b antibody [EPR6995] (ab134087) at 1/10000 dilution + Human platelets at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 69 kDaDiluting and blocking buffer: 5% NFDM/TBST
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ab134087 staining CD42b in human bone marrow tissue by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraffin and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Negative control 1: PBS in place of primary antibody.
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Anti-CD42b antibody [EPR6995] (ab134087) at 1/10000 dilution + Human platelet lysate at 10 µg
Secondary
HRP labelled Goat anti-rabbit at 1/2000 dilution
Predicted band size: 69 kDa
Observed band size: 143 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab134087 has been referenced in 13 publications.
- Sun YE et al. Different Thrombus Histology in a Cancer Patient with Deep Vein Thrombosis and Recurrent Strokes. J Stroke 24:300-302 (2022). PubMed: 35677986
- Yu H et al. Platelet biomarkers for a descending cognitive function: A proteomic approach. Aging Cell 20:e13358 (2021). PubMed: 33942972
- Zhang P CD42b Immunostaining as a Marker for Placental Fibrinoid in Normal Pregnancy and Complications. Fetal Pediatr Pathol 40:387-394 (2021). PubMed: 31986954
- Feng FE et al. HCMV modulates c-Mpl/IEX-1 pathway-mediated megakaryo/thrombopoiesis via PDGFRα and αvβ3 receptors after allo-HSCT. J Cell Physiol 236:6726-6741 (2021). PubMed: 33611789
- Park H et al. Histological features of intracranial thrombi in stroke patients with cancer. Ann Neurol 86:143-149 (2019). PubMed: 31025392