Anti-Poly (ADP-Ribose) Polymer antibody [10H] (ab14459)
Key features and details
- Mouse monoclonal [10H] to Poly (ADP-Ribose) Polymer
- Suitable for: IHC-Fr
- Reacts with: Species independent
- Isotype: IgG3
Overview
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Product name
Anti-Poly (ADP-Ribose) Polymer antibody [10H]
See all Poly (ADP-Ribose) Polymer primary antibodies -
Description
Mouse monoclonal [10H] to Poly (ADP-Ribose) Polymer -
Host species
Mouse -
Specificity
This antibody reacts with poly (ADP-Ribose) Polymer synthesized by a variety of poly(ADP- ribose) polymerases (PARP)-related enzymes including PARP1, 2, 3, tankyrase, vPARP, sPARP and others. The antibody does not cross-react with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA. -
Tested applications
Suitable for: IHC-Frmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Other Immunogen Type conjugated to bovine serum albumin. Poly (ADP-Ribose) polymer mixed with methylated bovine serum albumin.
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Positive control
- Rat liver induced for Poly (ADP-Ribose) Polymer synthesis by injection with diethylnitrosamine. MEF's treated with 500um Hydrogen Peroxide.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.242% Tris, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
10H -
Myeloma
NS1 -
Isotype
IgG3 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
Target
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Relevance
Poly (ADP-Ribose) is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerases (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer poly (ADP-Ribose), with chain lengths ranging from 2 to 300 residues, containing approximately 2% branching in the chain. Poly (ADP-Ribose) polymer becomes attached to nuclear proteins, and to PARP itself (automodification). Under normal conditions, cells display low basal level of poly (ADP-Ribose) polymer, which can dramatically increase in cells exposed to DNA damaging agents (irradiation, alkylation, etc.). This increase of polymer synthesis is usually transient and is followed by a rapid degradation phase with a short half life which can be less than 1 min. The low endogenous level of polymer in unstimulated cells and its rapid catabolism during DNA damage has been ascribed to high activity of the polymer catabolizing enzyme poly(ADP-ribose) glycohydrolyase (PARG). -
Alternative names
- pADPr antibody
- Poly ADP ribose antibody
Images
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Immunohistochemistry of rat livers treated with diethylnitrosamine (200 mg/kg) and stained with ab14459 diluted 1/100. After treatment livers were removed and rapidly processed 10 hr later, at peak polymer induction. Left hand side image was from diethylnitrosamine untreated liver tissue and right one represents DEN treated sections.
Datasheets and documents
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SDS download
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Datasheet download
References (56)
ab14459 has been referenced in 56 publications.
- Morgan SM et al. The three-dimensional structure of Epstein-Barr virus genome varies by latency type and is regulated by PARP1 enzymatic activity. Nat Commun 13:187 (2022). PubMed: 35039491
- Jhanji M et al. Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration. Nat Commun 13:3244 (2022). PubMed: 35688816
- Santofimia-Castaño P et al. NUPR1 protects against hyperPARylation-dependent cell death. Commun Biol 5:732 (2022). PubMed: 35869257
- Zhang W et al. Role of PARP-1 in Human Cytomegalovirus Infection and Functional Partners Encoded by This Virus. Viruses 14:N/A (2022). PubMed: 36146855
- Luo Q et al. OTUD1 Activates Caspase-Independent and Caspase-Dependent Apoptosis by Promoting AIF Nuclear Translocation and MCL1 Degradation. Adv Sci (Weinh) 8:2002874 (2021). PubMed: 33898171