Recombinant human PARP2 protein (ab198766)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 12% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human PARP2 protein
See all PARP2 proteins and peptides -
Biological activity
Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Assay Conditions: Enzyme reaction is conducted using a PARP2 Assay Kit at room temperature for 1 hour.
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Purity
>= 12 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
AARRRRSTGGGRARALNESKRVNNGNTAPEDSSPAKKTRRCQRQESKKMP VAGGKANKDRTEDKQDGMPGRSWASKRVSESVKALLLKGKAPVDPECTAK VGKAHVYCEGNDVYDVMLNQTNLQFNNNKYYLIQLLEDDAQRNFSVWMRW GRVGKMGQHSLVACSGNLNKAKEIFQKKFLDKTKNNWEDREKFEKVPGKY DMLQMDYATNTQDEEETKKEESLKSPLKPESQLDLRVQELIKLICNVQAM EEMMMEMKYNTKKAPLGKLTVAQIKAGYQSLKKIEDCIRAGQHGRALMEA CNEFYTRIPHDFGLRTPPLIRTQKELSEKIQLLEALGDIEIAIKLVKTEL QSPEHPLDQHYRNLHCALRPLDHESYEFKVISQYLQSTHAPTHSDYTMTL LDLFEVEKDGEKEAFREDLHNRMLLWHGSRMSNWVGILSHGLRIAPPEAP ITGYMFGKGIYFADMSSKSANYCFASRLKNTGLLLLSEVALGQCNELLEA NPKAEGLLQGKHSTKGLGKMAPSSAHFVTLNGSTVPLGPASDTGILNPDG YTLNYNEYIVYNPNQVRMRYLLKVQFNFLQLW -
Predicted molecular weight
92 kDa including tags -
Amino acids
2 to 583 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_005484.
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Specifications
Our Abpromise guarantee covers the use of ab198766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.49% Glutathione, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- ADP ribosyltransferase like 2
- ADP-ribosyltransferase diphtheria toxin-like 2
- ADPRT 2
see all -
Function
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. -
Tissue specificity
Widely expressed, mainly in actively dividing tissues. The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected in kidney, liver, lung, placenta, ovary and spleen; levels are low in leukocytes, colon, small intestine, prostate and thymus. -
Sequence similarities
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain. -
Post-translational
modificationsPoly-ADP-ribosylated by PARP1. -
Cellular localization
Nucleus. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab198766 has been referenced in 1 publication.
- Müller KH et al. Poly(ADP-Ribose) Links the DNA Damage Response and Biomineralization. Cell Rep 27:3124-3138.e13 (2019). PubMed: 31189100