Anti-RECA1 antibody [HIS52] (ab22492)
Key features and details
- Mouse monoclonal [HIS52] to RECA1
- Suitable for: ICC/IF, IHC-P, IHC-Fr
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-RECA1 antibody [HIS52] -
Description
Mouse monoclonal [HIS52] to RECA1 -
Host species
Mouse -
Specificity
This antibody recognises RecA-1, a cell surface antigen which is expressed by all rat endothelial cells. -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Rat
Does not react with: Mouse, Sheep, Rabbit, Goat, Chicken, Guinea pig, Pig -
Immunogen
Tissue, cells or virus corresponding to Rat RECA1. Stromal cells from rat lymph node
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
HIS52 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
Fix with acetone. |
Notes |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. Fix with acetone. |
Target
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Relevance
RECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognises a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens.
Protocols
Datasheets and documents
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Datasheet download
References (15)
ab22492 has been referenced in 15 publications.
- Pritchard KA et al. Role of endoplasmic reticulum stress in impaired neonatal lung growth and bronchopulmonary dysplasia. PLoS One 17:e0269564 (2022). PubMed: 36018859
- Zhao Y et al. Activated mesangial cells induce glomerular endothelial cells proliferation in rat anti-Thy-1 nephritis through VEGFA/VEGFR2 and Angpt2/Tie2 pathway. Cell Prolif 54:e13055 (2021). PubMed: 33987885
- Xu ZX et al. Histological and functional outcomes in a rat model of hemisected spinal cord with sustained VEGF/NT-3 release from tissue-engineered grafts. Artif Cells Nanomed Biotechnol 48:362-376 (2020). PubMed: 31899965
- Kumar H et al. Elevated TRPV4 Levels Contribute to Endothelial Damage and Scarring in Experimental Spinal Cord Injury. J Neurosci 40:1943-1955 (2020). PubMed: 31974206
- Tabibzadeh N et al. Plasma heme-induced renal toxicity is related to a capillary rarefaction. Sci Rep 7:40156 (2017). PubMed: 28071761