Anti-Integrin beta 1d antibody [2B1] (ab8991)
Key features and details
- Mouse monoclonal [2B1] to Integrin beta 1d
- Suitable for: WB, IHC
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Integrin beta 1d antibody [2B1] -
Description
Mouse monoclonal [2B1] to Integrin beta 1d -
Host species
Mouse -
Specificity
Recognizes specifically the cytoplasmic domain of integrin subunit beta 1D present in cardiac and skeletal muscle.
A broad species reactivity is expected because of the conserved nature of the epitope.
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Tested applications
Suitable for: WB, IHCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Integrin beta 1d. The immunogen corresponds to the C-terminal 24 amino acids of integrin ß1D including an appending N-terminal cysteine (
CQENPIYKS-PINNFKNPNYGRKAGL
) coupled to keyhole limpet hemocyanin.
Database link: P05556 -
Epitope
Cytoplasmic domain, C-terminal. Isoform of the Integrin b1 subunit present in cardiac and skeletal muscle. -
Positive control
- WB: Human heart, tongue and skeletal muscle tissue lysates. IHC: Human myocardium.
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General notes
The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an alpha and a beta subunit in interconnecting the cytoskeleton and the extracellular matrix.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an alpha and a beta subunit in interconnecting the cytoskeleton and the extracellular matrix. -
Clonality
Monoclonal -
Clone number
2B1 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/100 - 1/1000.
|
|
IHC |
1/25 - 1/200.
|
Notes |
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WB
1/100 - 1/1000. |
IHC
1/25 - 1/200. |
Target
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Relevance
Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. The isoform beta-1D of Integrin 1, is expressed specifically in striated muscle (skeletal and cardiac muscle). -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 3688 Human
- Omim: 135630 Human
- SwissProt: P05556 Human
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Alternative names
- CD29 antibody
- FNRB antibody
- Integrin beta 1 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab8991 has been referenced in 8 publications.
- Bugiardini E et al. Integrin α7 Mutations Are Associated With Adult-Onset Cardiac Dysfunction in Humans and Mice. J Am Heart Assoc 11:e026494 (2022). PubMed: 36444867
- Ma HY et al. The progression of intracerebral hemorrhage (ICH) is related to the expression of integrin ?1 (ITGB1). Chin Neurosurg J 7:14 (2021). PubMed: 33641668
- Kanoldt V et al. Metavinculin modulates force transduction in cell adhesion sites. Nat Commun 11:6403 (2020). PubMed: 33335089
- Yuan T et al. Low-density lipoprotein receptor-related protein 6 regulates alternative pre-mRNA splicing. J Cell Mol Med 22:4653-4663 (2018). PubMed: 30070011
- Lee HJ et al. Dysregulation of nuclear receptor COUP-TFII impairs skeletal muscle development. Sci Rep 7:3136 (2017). WB, IF ; Mouse . PubMed: 28600496
- Barton ER et al. Deletion of muscle GRP94 impairs both muscle and body growth by inhibiting local IGF production. FASEB J : (2012). ICC/IF ; Mouse . PubMed: 22649033
- Barton ER Restoration of gamma-sarcoglycan localization and mechanical signal transduction are independent in murine skeletal muscle. J Biol Chem 285:17263-70 (2010). WB ; Mouse . PubMed: 20371873
- Evans M et al. Expression profiling reveals heightened apoptosis and supports fiber size economy in the murine muscles of mastication. Physiol Genomics 35:86-95 (2008). PubMed: 18593863