Overview

  • Product name

    Anti-ProDynorphin antibody
    See all ProDynorphin primary antibodies
  • Description

    Rabbit polyclonal to ProDynorphin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 143-192 (SELMRDAQLN DGAMETGTLY LAEEDPKEQV KRYGGFLRKY PKRSSEVAGE) of Human ProDynorphin (NP_077722).

  • Positive control

    • HeLa cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.
    Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opiod activity, it is 700 times more potent than Leu-enkephalin.
    Leumorphin has a typical opiod activity and may have anti-apoptotic effect.
  • Involvement in disease

    Defects in PDYN are the cause of spinocerebellar ataxia type 23 (SCA23) [MIM:610245]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA23 is an adult-onset autosomal dominant form characterized by slowly progressive gait and limb ataxia, with variable additional features, including peripheral neuropathy and dysarthria.
  • Sequence similarities

    Belongs to the opioid neuropeptide precursor family.
  • Post-translational
    modifications

    The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADCA antibody
    • Alpha neoendorphin antibody
    • Beta neoendorphin antibody
    • Beta-neoendorphin-dynorphin antibody
    • Big Dyn antibody
    • Big dynorphin antibody
    • Dyn antibody
    • Dyn-A17 antibody
    • Dyn-B antibody
    • Dynorphin A antibody
    • Dynorphin A(1-13) antibody
    • Dynorphin A(1-17) antibody
    • Dynorphin A(1-8) antibody
    • Dynorphin B antibody
    • Dynorphin B(1-13) antibody
    • Dynorphin B-29 antibody
    • Enkephalin B antibody
    • Leu enkephalin antibody
    • Leumorphin antibody
    • Neoendorphin dynorphin enkephalin prepropeptide antibody
    • PDYN antibody
    • PDYN_HUMAN antibody
    • PENKB antibody
    • Preprodynorphin antibody
    • Preproenkephalin B antibody
    • Prodynorphin antibody
    • Proenkephalin B antibody
    • Rimorphin antibody
    • SCA23 antibody
    see all

Images

  • Anti-ProDynorphin antibody (ab102568) at 1 µg/ml + Hela cell lysate at 10 µg

    Predicted band size: 28 kDa



    Gel concentration: 12%

References

ab102568 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Non-Denaturing (Native) (12%)
Loading amount
12 µg
Treatment
Lysis Buffer and Protease and Phosphatase Inhibitors
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Marta Portero

Verified customer

Submitted Jun 09 2016

Question
Answer

Thank you for contacting us.
From the sequence of the peptide used to raise ab102568 antibody, it is 100% homologous only to human PDYN (http://www.uniprot.org/uniprot/P01213). The larger sized band must therefore be a complex of proteins that includes PDYN (although this is a denatured, reduced sample making this an unlikely case) or a unspecific band.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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