Recombinant Anti-Proenkephalin-B antibody [EPR24508-78] - BSA and Azide free (ab283514)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24508-78] to ProDynorphin - BSA and Azide free
- Suitable for: IHC-P, WB, IP, Dot blot
- Reacts with: Rat
Related conjugates and formulations
Overview
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Product name
Anti-Proenkephalin-B antibody [EPR24508-78] - BSA and Azide free
See all ProDynorphin primary antibodies -
Description
Rabbit monoclonal [EPR24508-78] to ProDynorphin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IP, Dot blotmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF or IHC-Fr -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat pituitary tissue lysate; LIF/His-rat Prodynorphin full-length protein, His-tagged rat Prodynorphin pro-peptide. IHC-P: Rat hippocampus and pituitary tissue. IP: Rat pituitary tissue lysate.
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General notes
ab283514 is the carrier-free version of ab283501.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24508-78 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab283514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Dot blot |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
Target
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Function
Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.
Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opiod activity, it is 700 times more potent than Leu-enkephalin.
Leumorphin has a typical opiod activity and may have anti-apoptotic effect. -
Involvement in disease
Defects in PDYN are the cause of spinocerebellar ataxia type 23 (SCA23) [MIM:610245]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA23 is an adult-onset autosomal dominant form characterized by slowly progressive gait and limb ataxia, with variable additional features, including peripheral neuropathy and dysarthria. -
Sequence similarities
Belongs to the opioid neuropeptide precursor family. -
Post-translational
modificationsThe N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 29190 Rat
- SwissProt: P06300 Rat
- Unigene: 44471 Rat
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Alternative names
- ADCA antibody
- Alpha neoendorphin antibody
- Beta neoendorphin antibody
see all
Images
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This data was developed using ab283501, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labelling ProDynorphin with ab283501 at 1/4000 (0.137 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat hippocampus (PMID: 16966485). The section was incubated with ab283501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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All lanes : Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501) at 1/1000 dilution
Lane 1 : Rat pituitary tissue lysate at 20 µg
Lane 2 : Rat liver tissue lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 18,23,28 kDa why is the actual band size different from the predicted?This data was developed using ab283501, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The observed molecular weight is consistent with the literature (PMID: 9712897).
Negative control: Rat liver (PMID:23649822).
Exposure time: 26 seconds.
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This data was developed using ab283501, the same antibody clone in a different buffer formulation.
ProDynorphin was immunoprecipitated from 0.35 mg Rat pituitary tissue lysate with ab283501 at 1/30 dilution (2 ug in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab283501 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat pituitary tissue lysate 10 ug
Lane 2: ab283501 IP in Rat pituitary tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab283501 in rat pituitary tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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This data was developed using ab283501, the same antibody clone in a different buffer formulation.
Dot blot analysis of ProDynorphin using ab283501 at 1/1000 (0.583 ug/ml) diultion followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.
Exposure time: 3 minutes.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-Proenkephalin-B antibody [EPR24508-78] (ab283501) at 1/1000 dilution
Lane 1 : LIF/His-rat ProDynorphin full-length protein
Lane 2 : His-tagged rat ProDynorphin pro-peptide
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 18,64 kDa why is the actual band size different from the predicted?This data was developed using ab283501, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
-
This data was developed using ab283501, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat pituitary tissue labelling ProDynorphin with ab283501 at 1/4000 (0.137 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat pituitary (PMID: 16966485). The section was incubated with ab283501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
This data was developed using ab283501, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labelling ProDynorphin with ab283501 at 1/4000 (0.137 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining on rat liver (PMID 23649822). The section was incubated with ab283501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab283514 has not yet been referenced specifically in any publications.