Product nameAnti-Profilin 1 antibody - Carboxyterminal end
See all Profilin 1 primary antibodies
DescriptionRabbit polyclonal to Profilin 1 - Carboxyterminal end
Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human
- Recombinant Human Profilin 1 protein (ab87760) can be used as a positive control in WB. Immunoblotting: whole cell extract of human HeLa cells, Immunoprecipitation: Mouse NIH-3T3 cell lysates Indirect Immunofluorescence: rat NRK cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab50667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 - 20 µg/ml.|
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).|
|IP||Use at an assay dependent concentration.|
FunctionBinds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
Sequence similaritiesBelongs to the profilin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Actin binding protein antibody
- ALS18 antibody
- Epididymis tissue protein Li 184a antibody
Anti-Profilin 1 antibody - Carboxyterminal end (ab50667) at 1/1000 dilution + Human platelet lysate at 20 µg
HRP-conjugated goat anti-rabbit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Lane 1: Positve control — Western blot: ab50667 at a concentration of 1μg/ml on whole extract of Mouse 3T3 cells at 10μg/ml.
Lane 2: Immunoprecipitation — 10 μg of ab50667 was used to immunoprecipitate Mouse Profilin 1 from 3T3 cell lysate (20μg/ml). Detection antibody: Rabbit Anti-Profilin 1 (N-terminal) antibody.
Lane 3: Negative control — non-relevant antibody was used for IP. Detection antibody: Rabbit Anti-Profilin 1 (N-terminal) antibody
ab50667 detecting Profilin 1 in Human platelets by Flow Cytometry. Platelets were isolated by spinning Platelet rich plasma on Histopaque. Cells were fixed in PFA and permeabilized in 0.1% Triton-X100 in 2% BSA for 30 minutes. The cell population was gated on platelets. The primary antibody was diluted 1/200 in 2% BSA in PBS and incubated with the sample for 18 hours at 4°C. An Alexa Fluor® 488-conjugated Chicken anti-Rabbit IgG (H+L) antibody, diluted 1/500 was used as the secondary antibody.
Abbreviations in the figure:
US Unstained, Red Peak;
IgG Rb: IgG Rabbit (Isotype Control), Blue Peak;
Profilin: Profilin 1, Green peak.
This product has been referenced in:
- Henty-Ridilla JL et al. Profilin Directly Promotes Microtubule Growth through Residues Mutated in Amyotrophic Lateral Sclerosis. Curr Biol 27:3535-3543.e4 (2017). Read more (PubMed: 29129529) »
- Guo Z et al. A Legionella effector modulates host cytoskeletal structure by inhibiting actin polymerization. Microbes Infect N/A:N/A (2013). Read more (PubMed: 24286927) »