Overview

  • Product name

    Anti-Profilin 1 antibody [EPR6303]
    See all Profilin 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6303] to Profilin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide orresponding to residues in the Human Profilin 1 protein (P07737).

  • Positive control

    • Jurkat, JAR, HepG2, and 293T cell lysates; and Human kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    Images

    • All lanes : Anti-Profilin 1 antibody [EPR6303] (ab133529) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 12 kDa

    • Immunohistochemistry analysis of Paraffin Embedded Human kidney tissue labelling Profilin 1 with ab133529 at 1/250.

    References

    This product has been referenced in:

    • Shen K  et al. Guttiferone K suppresses cell motility and metastasis of hepatocellular carcinoma by restoring aberrantly reduced profilin 1. Oncotarget 7:56650-56663 (2016). WB, IHC . Read more (PubMed: 27494863) »
    • Cheng YJ  et al. Silencing profilin-1 inhibits gastric cancer progression via integrin ß1/focal adhesion kinase pathway modulation. World J Gastroenterol 21:2323-35 (2015). WB ; Human . Read more (PubMed: 25741138) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133529.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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