Recombinant Anti-Profilin 1 antibody [EPR6303] (ab133529)
![Western blot - Anti-Profilin 1 antibody [EPR6303] (ab133529)](https://www.abcam.com/ps/products/133/ab133529/Images/ab133529-365775-anti-profilin-1-antibody-epr6303-western-blot-knockout-hap1-pfn1-hela-jurkat-human-lysates.jpg "Western blot - Anti-Profilin 1 antibody [EPR6303] (ab133529)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6303] to Profilin 1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Profilin 1 antibody [EPR6303]
See all Profilin 1 primary antibodies -
Description
Rabbit monoclonal [EPR6303] to Profilin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide orresponding to residues in the Human Profilin 1 protein (P07737).
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Positive control
- Jurkat, JAR, HepG2, and 293T cell lysates; and Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6303 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 12 kDa. | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. -
Sequence similarities
Belongs to the profilin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 5216 Human
- Entrez Gene: 18643 Mouse
- Entrez Gene: 64303 Rat
- Omim: 176610 Human
- SwissProt: P07737 Human
- SwissProt: P62962 Mouse
- SwissProt: P62963 Rat
- Unigene: 494691 Human
see all -
Alternative names
- Actin binding protein antibody
- ALS18 antibody
- Epididymis tissue protein Li 184a antibody
see all
Images
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Western blot - Anti-Profilin 1 antibody [EPR6303] (ab133529)All lanes : Anti-Profilin 1 antibody [EPR6303] (ab133529) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PFN1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 12 kDaLanes 1 - 4: Merged signal (red and green). Green - ab133529 observed at 15 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133529 was shown to specifically react with in wild-type HAP1 cells as signal was lost in PFN1 knockout cells. Wild-type and PFN1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab133529 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [EPR6303] (ab133529)](https://www.abcam.com/ps/products/133/ab133529/Images/ab133529-147237-anti-profilin-1-antibody-epr6303-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [EPR6303] (ab133529)Immunohistochemistry analysis of Paraffin Embedded Human kidney tissue labelling Profilin 1 with ab133529 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Profilin 1 antibody [EPR6303] (ab133529)](https://www.abcam.com/ps/products/133/ab133529/Images/ab133529-147238-anti-profilin-1-antibody-epr6303-western-blot.jpg)
Western blot - Anti-Profilin 1 antibody [EPR6303] (ab133529)All lanes : Anti-Profilin 1 antibody [EPR6303] (ab133529) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : JAR cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 12 kDa -
![Anti-Profilin 1 antibody [EPR6303] (ab133529)](https://www.abcam.com/ps/products/133/ab133529/Images/ab133529-4-benefits-of-recombinant-antibodies.png)
Anti-Profilin 1 antibody [EPR6303] (ab133529)
Protocols
Datasheets and documents
References (3)
ab133529 has been referenced in 3 publications.
- Merlotti D et al. Mutation of PFN1 Gene in an Early Onset, Polyostotic Paget-like Disease. J Clin Endocrinol Metab 105:N/A (2020). PubMed: 32392277
- Shen K et al. Guttiferone K suppresses cell motility and metastasis of hepatocellular carcinoma by restoring aberrantly reduced profilin 1. Oncotarget 7:56650-56663 (2016). WB, IHC . PubMed: 27494863
- Cheng YJ et al. Silencing profilin-1 inhibits gastric cancer progression via integrin ß1/focal adhesion kinase pathway modulation. World J Gastroenterol 21:2323-35 (2015). WB ; Human . PubMed: 25741138
