Recombinant
Recombinant Anti-Profilin 1 antibody [EPR6303] (ab133529)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-Profilin 1 antibody [EPR6303]
See all Profilin 1 primary antibodies -
Description
Rabbit monoclonal [EPR6303] to Profilin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide orresponding to residues in the Human Profilin 1 protein (P07737).
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Positive control
- Jurkat, JAR, HepG2, and 293T cell lysates; and Human kidney tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6303 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 12 kDa. | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Application notes
Is unsuitable for Flow Cyt,ICC or IP.
Target
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Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. -
Sequence similarities
Belongs to the profilin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 5216 Human
- Entrez Gene: 18643 Mouse
- Entrez Gene: 64303 Rat
- Omim: 176610 Human
- SwissProt: P07737 Human
- SwissProt: P62962 Mouse
- SwissProt: P62963 Rat
- Unigene: 494691 Human
see all -
Alternative names
- Actin binding protein antibody
- ALS18 antibody
- Epididymis tissue protein Li 184a antibody
see all
Images
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![Western blot - Anti-Profilin 1 antibody [EPR6303] (ab133529)](https://www.abcam.com/ps/products/133/ab133529/Images/ab133529-147238-anti-profilin-1-antibody-epr6303-western-blot.jpg)
Western blot - Anti-Profilin 1 antibody [EPR6303] (ab133529)All lanes : Anti-Profilin 1 antibody [EPR6303] (ab133529) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : JAR cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 12 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [EPR6303] (ab133529)](https://www.abcam.com/ps/products/133/ab133529/Images/ab133529-147237-anti-profilin-1-antibody-epr6303-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [EPR6303] (ab133529)Immunohistochemistry analysis of Paraffin Embedded Human kidney tissue labelling Profilin 1 with ab133529 at 1/250.
Datasheets and documents
References
This product has been referenced in:
- Shen K et al. Guttiferone K suppresses cell motility and metastasis of hepatocellular carcinoma by restoring aberrantly reduced profilin 1. Oncotarget 7:56650-56663 (2016). WB, IHC . Read more (PubMed: 27494863) »
- Cheng YJ et al. Silencing profilin-1 inhibits gastric cancer progression via integrin ß1/focal adhesion kinase pathway modulation. World J Gastroenterol 21:2323-35 (2015). WB ; Human . Read more (PubMed: 25741138) »
