Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Profilin 1 antibody [EPR6303] (ab133529)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6303] to Profilin 1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Profilin 1 antibody [EPR6303]
    See all Profilin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6303] to Profilin 1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide orresponding to residues in the Human Profilin 1 protein (P07737).

  • Positive control

    • Jurkat, JAR, HepG2, and 293T cell lysates; and Human kidney tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab133529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    Images

    • Western blot - Anti-Profilin 1 antibody [EPR6303] (ab133529)
      Western blot - Anti-Profilin 1 antibody [EPR6303] (ab133529)
      All lanes : Anti-Profilin 1 antibody [EPR6303] (ab133529) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : PFN1 knockout HAP1 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : Jurkat whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 12 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab133529 observed at 15 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab133529 was shown to specifically react with in wild-type HAP1 cells as signal was lost in PFN1 knockout cells. Wild-type and PFN1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab133529 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [EPR6303] (ab133529)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [EPR6303] (ab133529)

      Immunohistochemistry analysis of Paraffin Embedded Human kidney tissue labelling Profilin 1 with ab133529 at 1/250.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-Profilin 1 antibody [EPR6303] (ab133529)
      Western blot - Anti-Profilin 1 antibody [EPR6303] (ab133529)
      All lanes : Anti-Profilin 1 antibody [EPR6303] (ab133529) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 12 kDa

    References

    Publishing research using ab133529? Please let us know so that we can cite the reference in this datasheet.

    ab133529 has been referenced in 3 publications.

    • Merlotti D  et al. Mutation of PFN1 Gene in an Early Onset, Polyostotic Paget-like Disease. J Clin Endocrinol Metab 105:N/A (2020). PubMed: 32392277
    • Shen K  et al. Guttiferone K suppresses cell motility and metastasis of hepatocellular carcinoma by restoring aberrantly reduced profilin 1. Oncotarget 7:56650-56663 (2016). WB, IHC . PubMed: 27494863
    • Cheng YJ  et al. Silencing profilin-1 inhibits gastric cancer progression via integrin ß1/focal adhesion kinase pathway modulation. World J Gastroenterol 21:2323-35 (2015). WB ; Human . PubMed: 25741138

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