Recombinant
RabMAb

Anti-Profilin 1 antibody [EPR6304] (ab124904)

Abreviews (2) Submit a Question Specific References (3)

Overview

  • Product name
    Anti-Profilin 1 antibody [EPR6304]
    See all Profilin 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6304] to Profilin 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Profilin 1 aa 100-200 (C terminal). The exact sequence is proprietary.

  • Positive control
    • Jurkat, JAR, HepG2 and HEK-293T cell lysates; Human colon tissue; HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 12 kDa (predicted molecular weight: 15 kDa).
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/250 - 1/500.

Target

Images

  • Western blot - Anti-Profilin 1 antibody [EPR6304] (ab124904)
    Western blot - Anti-Profilin 1 antibody [EPR6304] (ab124904)
    All lanes : Anti-Profilin 1 antibody [EPR6304] (ab124904) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : JAR cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 15 kDa
    Observed band size: 12 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [EPR6304] (ab124904)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [EPR6304] (ab124904)

    ab124904, at 1/500 dilution, staining Profilin 1 in paraffin-embedded human colon tissue by Immunohistochemistry.

  • Immunocytochemistry/ Immunofluorescence - Anti-Profilin 1 antibody [EPR6304] (ab124904)
    Immunocytochemistry/ Immunofluorescence - Anti-Profilin 1 antibody [EPR6304] (ab124904)
    ab124904, at 1/250 dilution, staining Profilin 1 in HeLa cells by Immunofluorescence.
  • Flow Cytometry - Anti-Profilin 1 antibody [EPR6304] (ab124904)
    Flow Cytometry - Anti-Profilin 1 antibody [EPR6304] (ab124904)
    Overlay histogram showing HeLa cells stained with ab124904 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124904, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Other - Anti-Profilin 1 antibody [EPR6304] (ab124904)
    Other - Anti-Profilin 1 antibody [EPR6304] (ab124904)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:
  • Matsukawa K  et al. Familial Amyotrophic Lateral Sclerosis-linked Mutations in Profilin 1 Exacerbate TDP-43-induced Degeneration in the Retina of Drosophila melanogaster through an Increase in the Cytoplasmic Localization of TDP-43. J Biol Chem 291:23464-23476 (2016). Read more (PubMed: 27634045) »
  • Wong SW  et al. H2O 2 Exposure Affects Myotube Stiffness and Actin Filament Polymerization. Ann Biomed Eng 43:1178-88 (2015). Read more (PubMed: 25371376) »

See all 3 Publications for this product

Publishing research using ab124904? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

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Votes: Highest First

Immunohistochemistry (Frozen sections) abreview for Anti-Profilin 1 antibody [EPR6304]

 Excellent
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic Heart)
Permeabilization
Yes - Tween-20
Specification
Embryonic Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted Nov 01 2016

Western blot abreview for Anti-Profilin 1 antibody [EPR6304]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Oct 19 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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