Key features and details
- Rabbit polyclonal to Profilin 1 (phospho S138)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Profilin 1 (phospho S138) antibody
See all Profilin 1 primary antibodies
DescriptionRabbit polyclonal to Profilin 1 (phospho S138)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Profilin 1 (phospho S138).
Database link: P07737
- Jurkat cell lysate stimulated with calyculin A (100nM) for 30 min.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab215752 was cross-adsorbed to unphosphorylated Profilin (Ser-138) then affinity purified using Profilin (Ser-138) peptide (without carrier).
Our Abpromise guarantee covers the use of ab215752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 15 kDa.|
FunctionBinds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
Sequence similaritiesBelongs to the profilin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Actin binding protein antibody
- ALS18 antibody
- Epididymis tissue protein Li 184a antibody
Lanes 1-3 : anti-Profilin 1 (aa 132-140)
Lanes 4-6 : Anti-Profilin 1 (phospho S138) antibody (ab215752) at 1/1000 dilution
Lanes 1 & 4 : Jurkat cell lysate stimulated with calyculin A (100nM) for 30 min
Lanes 2 & 5 : Jurkat cell lysate stimulated with calyculin A (100nM) for 30 min with unphosphorylated Profilin 1
Lanes 3 & 6 : Jurkat cell lysate stimulated with calyculin A (100nM) for 30 min with Profilin 1 phospho S132 peptide
Predicted band size: 15 kDa
Observed band size: 15 kDa
ab215752 has not yet been referenced specifically in any publications.