Key features and details
- Rabbit polyclonal to Profilin 2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Profilin 2 antibody
See all Profilin 2 primary antibodies
DescriptionRabbit polyclonal to Profilin 2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Monkey
Synthetic peptide within Human Profilin 2 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P35080
- IHC-P: Human brain cortex tissue. WB: A431 and NCI-H1299 whole cell lysates; Mouse brain and skeletal muscle lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 15 kDa.|
FunctionBinds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
Tissue specificityHighly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver.
Sequence similaritiesBelongs to the profilin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- D3S1319E antibody
- PFL antibody
- PFN 2 antibody
Formalin-fixed, paraffin-embedded human brain cortex tissue stained for Profilin 2 using ab230673 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-Profilin 2 antibody (ab230673) at 1/500 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat skeletal muscle lysate
Predicted band size: 15 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230673 has not yet been referenced specifically in any publications.