Overview

  • Product name

    Anti-Profilin 2 antibody - C-terminal
    See all Profilin 2 primary antibodies
  • Description

    Rabbit polyclonal to Profilin 2 - C-terminal
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Cynomolgus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Profilin 2 aa 125-140 (C terminal).
    Sequence:

    NKKAYSMAKYLRDSGF


    Database link: P35080

  • Positive control

    • WB: human placenta tissue, rat brain tissue and mouse brain tissue; IHC-P: Human intestinal cancer tissue; FC: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab191054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue staining Profilin 2 with ab191054 at 1µg/ml, followed by a biotinylated goat anti-rabbit IgG secondary antibody. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

  • All lanes : Anti-Profilin 2 antibody - C-terminal (ab191054) at 0.5 µg/ml

    Lane 1 : Human placenta tissue lysates
    Lane 2 : Rat brain tissue lysates
    Lane 3 : Mouse brain tissue lysates

    Secondary
    All lanes : Goat anti-rabbit IgG-HRP at 1/10000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa

  • Immunohistochemical analysis of paraffin-embedded human intestincal cancer tissue staining Profilin 2 with ab191054 at 1µg/ml, followed by a biotinylated goat anti-rabbit IgG secondary antibody. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

  • Flow Cytometry analysis of A431 cells labeling Profilin 2 with ab191054 (1μg/1x106 cells), DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody (blue). Isotype control antibody was rabbit IgG (1μg/1x106) (green). An unlabelled samples was used as a second control (red). The cells were blocked with 10% normal goat serum

     

     

References

ab191054 has not yet been referenced specifically in any publications.

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