Key features and details
- Rabbit polyclonal to Profilin 2 - C-terminal
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Profilin 2 antibody - C-terminal
See all Profilin 2 primary antibodies
DescriptionRabbit polyclonal to Profilin 2 - C-terminal
Tested applicationsSuitable for: Flow Cyt, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Cynomolgus monkey, Orangutan
- WB: human placenta tissue, rat brain tissue and mouse brain tissue; IHC-P: Human intestinal cancer tissue; Flow Cyt: A431 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionBinds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
Tissue specificityHighly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver.
Sequence similaritiesBelongs to the profilin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- D3S1319E antibody
- PFL antibody
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue staining Profilin 2 with ab191054 at 1µg/ml, followed by a biotinylated goat anti-rabbit IgG secondary antibody. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
All lanes : Anti-Profilin 2 antibody - C-terminal (ab191054) at 0.5 µg/ml
Lane 1 : Human placenta tissue lysates
Lane 2 : Rat brain tissue lysates
Lane 3 : Mouse brain tissue lysates
All lanes : Goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Immunohistochemical analysis of paraffin-embedded human intestincal cancer tissue staining Profilin 2 with ab191054 at 1µg/ml, followed by a biotinylated goat anti-rabbit IgG secondary antibody. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Flow Cytometry analysis of A431 cells labeling Profilin 2 with ab191054 (1μg/1x106 cells), DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody (blue). Isotype control antibody was rabbit IgG (1μg/1x106) (green). An unlabelled samples was used as a second control (red). The cells were blocked with 10% normal goat serum
ab191054 has not yet been referenced specifically in any publications.