Recombinant Anti-Profilin 2 antibody [EPR11030] (ab174322)
![Western blot - Anti-Profilin 2 antibody [EPR11030] (ab174322)](https://www.abcam.com/ps/products/174/ab174322/Images/ab174322-199963-anti-profilin-2-antibody-epr11030-western-blot.jpg "Western blot - Anti-Profilin 2 antibody [EPR11030] (ab174322)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11030] to Profilin 2
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Profilin 2 antibody [EPR11030]
See all Profilin 2 primary antibodies -
Description
Rabbit monoclonal [EPR11030] to Profilin 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Profilin 2 aa 50 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P35080 -
Positive control
- Human fetal brain and MCF7 cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11030 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab174322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa). | |
| IP | 1/10 - 1/100. |
Target
-
Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. -
Tissue specificity
Highly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver. -
Sequence similarities
Belongs to the profilin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
-
Database links
- Entrez Gene: 5217 Human
- Entrez Gene: 18645 Mouse
- Entrez Gene: 81531 Rat
- Omim: 176590 Human
- SwissProt: P35080 Human
- SwissProt: Q9JJV2 Mouse
- SwissProt: Q9EPC6 Rat
- Unigene: 91747 Human
see all -
Alternative names
- D3S1319E antibody
- PFL antibody
- PFN 2 antibody
see all
Images
-
Western blot - Anti-Profilin 2 antibody [EPR11030] (ab174322)All lanes : Anti-Profilin 2 antibody [EPR11030] (ab174322) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : MCF7 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 15 kDa -
![Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] (ab174322)](https://www.abcam.com/ps/products/174/ab174322/Images/ab174322-199964-anti-profilin-2-antibody-epr11030-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] (ab174322)Western blot analysis on immunoprecipitation pellet from MCF7 cell lysate, immunoprecipitated using ab174322 at 1/10 dilution.
-
![Anti-Profilin 2 antibody [EPR11030] (ab174322)](https://www.abcam.com/ps/products/174/ab174322/Images/ab174322-3-benefits-of-recombinant-antibodies.png)
Anti-Profilin 2 antibody [EPR11030] (ab174322)
Protocols
Datasheets and documents
References (0)
ab174322 has not yet been referenced specifically in any publications.
