Recombinant Anti-Profilin 2 antibody [EPR11030] (ab174322)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Profilin 2 antibody [EPR11030]
See all Profilin 2 primary antibodies -
Description
Rabbit monoclonal [EPR11030] to Profilin 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Profilin 2 aa 50 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P35080 -
Positive control
- Human fetal brain and MCF7 cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11030 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab174322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa). | |
| IP | 1/10 - 1/100. |
Target
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Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. -
Tissue specificity
Highly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver. -
Sequence similarities
Belongs to the profilin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 5217 Human
- Entrez Gene: 18645 Mouse
- Entrez Gene: 81531 Rat
- Omim: 176590 Human
- SwissProt: P35080 Human
- SwissProt: Q9JJV2 Mouse
- SwissProt: Q9EPC6 Rat
- Unigene: 91747 Human
see all -
Alternative names
- D3S1319E antibody
- PFL antibody
- PFN 2 antibody
see all
Images
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![Western blot - Anti-Profilin 2 antibody [EPR11030] (ab174322)](https://www.abcam.com/ps/products/174/ab174322/Images/ab174322-199963-anti-profilin-2-antibody-epr11030-western-blot.jpg)
Western blot - Anti-Profilin 2 antibody [EPR11030] (ab174322)All lanes : Anti-Profilin 2 antibody [EPR11030] (ab174322) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : MCF7 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 15 kDa -
![Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] (ab174322)](https://www.abcam.com/ps/products/174/ab174322/Images/ab174322-199964-anti-profilin-2-antibody-epr11030-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] (ab174322)Western blot analysis on immunoprecipitation pellet from MCF7 cell lysate, immunoprecipitated using ab174322 at 1/10 dilution.
Datasheets and documents
References
ab174322 has not yet been referenced specifically in any publications.
