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  1. Link

    profilin-2-antibody-epr11030-ab174322.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Profilin 2 antibody [EPR11030] (ab174322)

  • Datasheet
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Western blot - Anti-Profilin 2 antibody [EPR11030] (ab174322)
  • Western blot - Anti-Profilin 2 antibody [EPR11030] (ab174322)
  • Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] (ab174322)
  • Anti-Profilin 2 antibody [EPR11030] (ab174322)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11030] to Profilin 2
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Recombinant Human Profilin 2 protein (ab185845)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
Product image
Human PFN2 (Profilin 2) knockout HeLa cell lysate (ab258108)

View more associated products

Overview

  • Product name

    Anti-Profilin 2 antibody [EPR11030]
    See all Profilin 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11030] to Profilin 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain and MCF7 cell lysates. WB: Wild-type HeLa cell lysate. Daudi cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11030
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly

Associated products

  • Alternative Versions

    • Anti-Profilin 2 antibody [EPR11030] - BSA and Azide free (ab249818)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human PFN2 (Profilin 2) knockout HeLa cell line (ab265833)
  • KO cell lysates

    • Human PFN2 (Profilin 2) knockout HeLa cell lysate (ab258108)
  • Positive Controls

    • MCF7 whole cell lysate (ab29537)
  • Recombinant Protein

    • Recombinant Human Profilin 2 protein (ab185845)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab174322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/5000. Detects a band of approximately 13 kDa (predicted molecular weight: 15 kDa).
IP
1/10 - 1/100.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Target

  • Function

    Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
  • Tissue specificity

    Highly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver.
  • Sequence similarities

    Belongs to the profilin family.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P35080 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5217 Human
    • Entrez Gene: 18645 Mouse
    • Entrez Gene: 81531 Rat
    • Omim: 176590 Human
    • SwissProt: P35080 Human
    • SwissProt: Q9JJV2 Mouse
    • SwissProt: Q9EPC6 Rat
    • Unigene: 91747 Human
    • Unigene: 271744 Mouse
    • Unigene: 203100 Rat
    see all
  • Alternative names

    • D3S1319E antibody
    • PFL antibody
    • PFN 2 antibody
    • Pfn2 antibody
    • PROF2_HUMAN antibody
    • Profilin II antibody
    • Profilin-2 antibody
    • Profilin2 antibody
    • ProfilinII antibody
    see all

Images

  • Western blot - Anti-Profilin 2 antibody [EPR11030] (ab174322)
    Western blot - Anti-Profilin 2 antibody [EPR11030] (ab174322)
    All lanes : Anti-Profilin 2 antibody [EPR11030] (ab174322) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PFN2 knockout HeLa cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 13 kDa why is the actual band size different from the predicted?



    False colour image of Western blot: Anti-Profilin 2 antibody [EPR11030] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab174322 was shown to bind specifically to Profilin 2. A band was observed at 13 kDa in wild-type HeLa cell lysates with no signal observed at this size in PFN2 knockout cell line ab265833 (knockout cell lysate ab258108). To generate this image, wild-type and PFN2 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

  • Western blot - Anti-Profilin 2 antibody [EPR11030] (ab174322)
    Western blot - Anti-Profilin 2 antibody [EPR11030] (ab174322)
    All lanes : Anti-Profilin 2 antibody [EPR11030] (ab174322) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : MCF7 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 15 kDa

  • Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] (ab174322)
    Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] (ab174322)

    Western blot analysis on immunoprecipitation pellet from MCF7 cell lysate, immunoprecipitated using ab174322 at 1/10 dilution.

  • Anti-Profilin 2 antibody [EPR11030] (ab174322)
    Anti-Profilin 2 antibody [EPR11030] (ab174322)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (1)

Publishing research using ab174322? Please let us know so that we can cite the reference in this datasheet.

ab174322 has been referenced in 1 publication.

  • Huang R  et al. NCAM regulates temporal specification of neural progenitor cells via profilin2 during corticogenesis. J Cell Biol 219:N/A (2020). PubMed: 31816056

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