Product nameAnti-Profilin antibody
See all Profilin primary antibodies
DescriptionRabbit polyclonal to Profilin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Species independent
Recombinant full length protein (His-tag) corresponding to Profilin aa 1-133. (Mercurialis annua; 6xHis-SUMO tag on N-terminus).
MSWQTYVDDHLMCDIDGQGQHLAAASIVGHDGSIWAQSASFPQLKPEEIT GIMKDFDEPGHLAPTGLYIAGTKYMVIQGESGAVIRGKKGSGGITIKKTG QLVFGIYEEPVTPGQCNMVVERLGDYLIEQGM
Database link: O49894
- WB: Recombinant Mercurialis annua Profilin protein with 6xHis-SUMO tag on N-terminus.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab225944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 31 kDa (predicted molecular weight: 14 kDa).|
RelevanceFunction: Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG (By similarity).
All lanes : Anti-Profilin antibody (ab225944) at 1/500 dilution
Lane 1 : Recombinant Mercurialis annua Profilin protein with 6xHis-SUMO tag on N-terminus at 0.08 µg
Lane 2 : Recombinant Mercurialis annua Profilin protein with 6xHis-SUMO tag on N-terminus at 0.04 µg
Lane 3 : Recombinant Mercurialis annua Profilin protein with 6xHis-SUMO tag on N-terminus at 0.02 µg
Lane 4 : Recombinant Mercurialis annua Profilin protein with 6xHis-SUMO tag on N-terminus at 0.01 µg
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
Predicted MW of recombinant protein: 31 kDa
ab225944 has not yet been referenced specifically in any publications.