Recombinant
RabMAb

Recombinant Anti-Progesterone antibody [EPPTX-R1] (ab215527)

Overview

  • Product name

    Anti-Progesterone antibody [EPPTX-R1]
    See all Progesterone primary antibodies
  • Description

    Rabbit monoclonal [EPPTX-R1] to Progesterone
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to Human Progesterone. The immunogen is 11 alpha-Hydroxyprogesterone hemisuccinyl-CH3 BSA.

  • Positive control

    • Competitive ELISA: Progesterone
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPPTX-R1
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab215527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Use 3-5 ng/ml.

Target

  • Relevance

    Progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Progesterone receptor, a member of the steroid receptor superfamily, mediates the physiologic effects of progesterone. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. Although PRA and PRB share several structural domains, they are distinct transcription factors that mediate their own response genes and physiologic effects with little overlap. It is composed of three domains: a modulating N terminal domain, a DNA binding domain and a C terminal steroid binding domain. Progesterone levels 1. men 30-60 pg/0.1ml 2. women pre ovulatory phase: 20-160 pg/0.1ml; ovulatory phase: 1,000-1,700 pg/0.1ml; post ovulatory phase: 1,000-1,700 pg/0.1ml; Pregnant: 16-18 weeks: 300-800 pg/0.1ml; 28-30 weeks: 6,500-14,700 pg/0.1ml; 38-40 weeks: 12,000-19,000 pg/0.1ml.
  • Cellular localization

    Secreted
  • Alternative names

    • Pregn 4 ene 3 20 dione antibody

Images

  • Goat anti-rabbit IgG was coated onto a 96-well plate. Serial dilution of Progesterone (0, 10, 25, 50, 100, 300, 1000 pg/ml) was added (50 μl). This was followed by adding 25 μl HRP-conjugated Progesterone and 3-5 ng/ml of ab215527 (50 μl) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing, 125 μl Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes at room temperature for color development. Then 125 ul of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 10 minutes. 

    This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

  • ab215527 is specific to Progesterone and the percent cross reactivity with other compounds is demonstrated in the table.

    This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

References

ab215527 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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