Recombinant Anti-Progesterone antibody [EPPTX-R1] - Low endotoxin, Azide free (ab222404)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTX-R1] to Progesterone - Low endotoxin, Azide free
- Suitable for: Competitive ELISA
- Reacts with: Species independent
Overview
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Product name
Anti-Progesterone antibody [EPPTX-R1] - Low endotoxin, Azide free
See all Progesterone primary antibodies -
Description
Rabbit monoclonal [EPPTX-R1] to Progesterone - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Competitive ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule. The immunogen is 11 alpha-Hydroxyprogesterone hemisuccinyl-CH3 BSA.
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Positive control
- Competitive ELISA: Progesterone
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General notes
ab222404 is the carrier-free version of ab215527.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPPTX-R1 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Competitive ELISA |
Use at an assay dependent concentration.
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Notes |
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Competitive ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Progesterone receptor, a member of the steroid receptor superfamily, mediates the physiologic effects of progesterone. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. Although PRA and PRB share several structural domains, they are distinct transcription factors that mediate their own response genes and physiologic effects with little overlap. It is composed of three domains: a modulating N terminal domain, a DNA binding domain and a C terminal steroid binding domain. Progesterone levels 1. men 30-60 pg/0.1ml 2. women pre ovulatory phase: 20-160 pg/0.1ml; ovulatory phase: 1,000-1,700 pg/0.1ml; post ovulatory phase: 1,000-1,700 pg/0.1ml; Pregnant: 16-18 weeks: 300-800 pg/0.1ml; 28-30 weeks: 6,500-14,700 pg/0.1ml; 38-40 weeks: 12,000-19,000 pg/0.1ml. -
Cellular localization
Secreted -
Alternative names
- Pregn 4 ene 3 20 dione antibody
Images
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This ELISA data used the same anti-Progesterone antibody clone [EPPTX-R1] in a different buffer formulation (cat# ab215527).
Goat anti-rabbit IgG was coated onto a 96-well plate. Serial dilution of Progesterone (0, 10, 25, 50, 100, 300, 1000 pg/ml) was added (50 μl). This was followed by adding 25 μl HRP-conjugated Progesterone and 3-5 ng/ml of ab215527 (50 μl) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing, 125 μl Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes at room temperature for color development. Then 125 ul of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 10 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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ab222404 is specific to Progesterone and the percent cross reactivity with other compounds is demonstrated in the table.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab222404 has not yet been referenced specifically in any publications.