Progesterone ELISA Kit (ab178652)
- Datasheet
- References
- Protocols
Overview
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Product name
Progesterone ELISA Kit
See all Progesterone kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Saliva 15 <=14% Inter-assay Sample n Mean SD CV% Saliva 12 <=10.1% -
Sample type
Saliva -
Assay type
Competitive -
Sensitivity
3.27 pg/ml -
Range
10 pg/ml - 5000 pg/ml -
Recovery
101.94 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Progesterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Progesterone in saliva.
A 96-well plate has been precoated with anti-Progesterone antibodies. Samples and the Progesterone-HRP conjugate are added to the wells, where Progesterone in the sample competes with the added Progesterone-HRP conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Progesterone in the sample and the intensity is measured at 450 nm.
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Tested applications
Suitable for: Competitive ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 40x Washing Solution 1 x 30ml Cover Foil 1 unit High Control 1 x 1ml Low Control 1 x 1ml Progesterone Coated Microplate (12 x 8 wells) 1 unit Progesterone HRP Conjugate 1 x 26ml Progesterone Standard 0 - 0 pg/mL 1 x 1ml Progesterone Standard 1 - 10 pg/mL 1 x 1ml Progesterone Standard 2 - 50 pg/mL 1 x 1ml Progesterone Standard 3 - 100 pg/mL 1 x 1ml Progesterone Standard 4 - 500 pg/mL 1 x 1ml Progesterone Standard 5 - 1000 pg/mL 1 x 1ml Progesterone Standard 6 - 5000 pg/mL 1 x 1ml Stop Solution 1 x 14ml Strip holder 1 unit TMB Substrate Solution 1 x 25ml -
Research areas
Applications
Our Abpromise guarantee covers the use of ab178652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Competitive ELISA | Use at an assay dependent concentration. |
Images
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Typical Standard CurveRepresentative standard curve using ab178652.
Protocols
References
ab178652 has not yet been referenced specifically in any publications.
