Anti-Progesterone Receptor antibody (ab63605)
Key features and details
- Rabbit polyclonal to Progesterone Receptor
- Suitable for: ICC/IF, ICC, ELISA, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Progesterone Receptor antibody
See all Progesterone Receptor primary antibodies -
Description
Rabbit polyclonal to Progesterone Receptor -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, ICC, ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic non-phosphopeptide derived from Human Progesterone Receptor around the phosphorylation site of serine 294 (G-R-SP-P-L).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab63605 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
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| ICC | (1) |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| IHC-P | (2) |
1/50 - 1/100.
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| Notes |
|---|
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ICC/IF
1/500 - 1/1000. |
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ICC
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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IHC-P
1/50 - 1/100. |
Target
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Function
The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Post-translational
modificationsPhosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation. -
Cellular localization
Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 5241 Human
- Entrez Gene: 18667 Mouse
- Omim: 607311 Human
- SwissProt: P06401 Human
- SwissProt: Q00175 Mouse
- Unigene: 32405 Human
- Unigene: 742403 Human
- Unigene: 12798 Mouse
see all -
Alternative names
- NR3C3 antibody
- Nuclear receptor subfamily 3 group C member 3 antibody
- PGR antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody (ab63605)
Paraffin-embedded human liver carcinoma tissue stained for Progesterone Receptor with ab63605 at 1/50 dilution in immunohistochemical analysis.
In the right-hand panel the sample is incubated with the immunizing peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody (ab63605)ab63605, at a 1/500 dilution, staining Human Progesterone Receptor in A549 cells, with (+) or without (-) immunising peptide, by Immunofluorescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody (ab63605)This image is courtesy of an Abreview submitted by Daniel Murtaab63605 staining Progesterone Receptor in Mouse uterus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 2% BSA for 1 hour at room temperature; antigen retrieval was by heat mediation in Tris_EDTA. Samples were incubated with primary antibody (1/100) for 10 hours at 4°C. A HRP-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab63605 has been referenced in 8 publications.
- Roßwag S et al. Functional Characterization of Circulating Tumor Cells (CTCs) from Metastatic ER+/HER2- Breast Cancer Reveals Dependence on HER2 and FOXM1 for Endocrine Therapy Resistance and Tumor Cell Survival: Implications for Treatment of ER+/HER2- Breast Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 33920089
- Hiraoka T et al. Differential roles of uterine epithelial and stromal STAT3 coordinate uterine receptivity and embryo attachment. Sci Rep 10:15523 (2020). PubMed: 32968170
- Pascual R et al. The RNA binding protein CPEB2 regulates hormone sensing in mammary gland development and luminal breast cancer. Sci Adv 6:eaax3868 (2020). PubMed: 32440535
- Pan B et al. Establishment and characterization of breast cancer organoids from a patient with mammary Paget's disease. Cancer Cell Int 20:365 (2020). PubMed: 32774159
- Matsuo M et al. Levonorgestrel Inhibits Embryo Attachment by Eliminating Uterine Induction of Leukemia Inhibitory Factor. Endocrinology 161:N/A (2020). PubMed: 31638694
- Yoshida K et al. Progesterone signaling during pregnancy in the lab opossum, Monodelphis domestica. Theriogenology 136:101-110 (2019). PubMed: 31254723
- Huang R et al. Androgen Receptor Expression and Bicalutamide Antagonize Androgen Receptor Inhibit ß-Catenin Transcription Complex in Estrogen Receptor-Negative Breast Cancer. Cell Physiol Biochem 43:2212-2225 (2017). PubMed: 29069648
- Bashour NM & Wray S Progesterone directly and rapidly inhibits GnRH neuronal activity via progesterone receptor membrane component 1. Endocrinology 153:4457-69 (2012). PubMed: 22822163
