Anti-Progesterone Receptor antibody (ab63605)
Key features and details
- Rabbit polyclonal to Progesterone Receptor
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Progesterone Receptor antibody
See all Progesterone Receptor primary antibodies -
Description
Rabbit polyclonal to Progesterone Receptor -
Host species
Rabbit -
Specificity
ab63605 detects endogenous levels of total Progesterone Receptor protein. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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Immunogen
Synthetic non-phosphopeptide derived from Human Progesterone Receptor around the phosphorylation site of serine 294 (G-R-SP-P-L).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
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Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab63605 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
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| IHC-P |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
|
|
| IHC-P | (1) |
1/50 - 1/100.
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| Notes |
|---|
|
ICC/IF
1/500 - 1/1000. |
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IHC-P
1/50 - 1/100. |
Target
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Function
The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Post-translational
modificationsPhosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation. -
Cellular localization
Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 5241 Human
- Omim: 607311 Human
- SwissProt: P06401 Human
- Unigene: 32405 Human
- Unigene: 742403 Human
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Alternative names
- NR3C3 antibody
- Nuclear receptor subfamily 3 group C member 3 antibody
- PGR antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody (ab63605)
Paraffin-embedded human liver carcinoma tissue stained for Progesterone Receptor with ab63605 at 1/50 dilution in immunohistochemical analysis.
In the right-hand panel the sample is incubated with the immunizing peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody (ab63605)ab63605, at a 1/500 dilution, staining Human Progesterone Receptor in A549 cells, with (+) or without (-) immunising peptide, by Immunofluorescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody (ab63605)This image is courtesy of an Abreview submitted by Daniel Murtaab63605 staining Progesterone Receptor in Mouse uterus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 2% BSA for 1 hour at room temperature; antigen retrieval was by heat mediation in Tris_EDTA. Samples were incubated with primary antibody (1/100) for 10 hours at 4°C. A HRP-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab63605 has been referenced in 5 publications.
- Hiraoka T et al. Differential roles of uterine epithelial and stromal STAT3 coordinate uterine receptivity and embryo attachment. Sci Rep 10:15523 (2020). PubMed: 32968170
- Pascual R et al. The RNA binding protein CPEB2 regulates hormone sensing in mammary gland development and luminal breast cancer. Sci Adv 6:eaax3868 (2020). PubMed: 32440535
- Yoshida K et al. Progesterone signaling during pregnancy in the lab opossum, Monodelphis domestica. Theriogenology 136:101-110 (2019). PubMed: 31254723
- Huang R et al. Androgen Receptor Expression and Bicalutamide Antagonize Androgen Receptor Inhibit ß-Catenin Transcription Complex in Estrogen Receptor-Negative Breast Cancer. Cell Physiol Biochem 43:2212-2225 (2017). PubMed: 29069648
- Bashour NM & Wray S Progesterone directly and rapidly inhibits GnRH neuronal activity via progesterone receptor membrane component 1. Endocrinology 153:4457-69 (2012). PubMed: 22822163