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    progesterone-receptor-antibody-ab63605.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-Progesterone Receptor antibody (ab63605)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (5)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody (ab63605)
  • Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody (ab63605)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody (ab63605)

Key features and details

  • Rabbit polyclonal to Progesterone Receptor
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Progesterone Receptor antibody
    See all Progesterone Receptor primary antibodies
  • Description

    Rabbit polyclonal to Progesterone Receptor
  • Host species

    Rabbit
  • Specificity

    ab63605 detects endogenous levels of total Progesterone Receptor protein.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Synthetic non-phosphopeptide derived from Human Progesterone Receptor around the phosphorylation site of serine 294 (G-R-SP-P-L).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab63605 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Progesterone
    • Neuroscience
    • Endocrine system
    • Gonadotrophic axis
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Progesterone
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Progesterone
    • Cancer
    • Tumor biomarkers
    • Receptors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Progesterone Receptor protein (ab159105)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab63605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
Application Abreviews Notes
ICC/IF
1/500 - 1/1000.
IHC-P (1)
1/50 - 1/100.
Notes
ICC/IF
1/500 - 1/1000.
IHC-P
1/50 - 1/100.

Target

  • Function

    The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
    Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
    Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications

    Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
    Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
    Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
  • Cellular localization

    Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
  • Target information above from: UniProt accession P06401 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5241 Human
    • Omim: 607311 Human
    • SwissProt: P06401 Human
    • Unigene: 32405 Human
    • Unigene: 742403 Human
    • Alternative names

      • NR3C3 antibody
      • Nuclear receptor subfamily 3 group C member 3 antibody
      • PGR antibody
      • PR antibody
      • PRA antibody
      • PRB antibody
      • PRGR_HUMAN antibody
      • Progesterone receptor antibody
      • Progestin receptor form A antibody
      • Progestin receptor form B antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody (ab63605)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody (ab63605)

      Paraffin-embedded human liver carcinoma tissue stained for Progesterone Receptor with ab63605 at 1/50 dilution in immunohistochemical analysis.

      In the right-hand panel the sample is incubated with the immunizing peptide.

    • Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody (ab63605)
      Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody (ab63605)
      ab63605, at a 1/500 dilution, staining Human Progesterone Receptor in A549 cells, with (+) or without (-) immunising peptide, by Immunofluorescence.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody (ab63605)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody (ab63605)This image is courtesy of an Abreview submitted by Daniel Murta

      ab63605 staining Progesterone Receptor in Mouse uterus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 2% BSA for 1 hour at room temperature; antigen retrieval was by heat mediation in Tris_EDTA. Samples were incubated with primary antibody (1/100) for 10 hours at 4°C. A HRP-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

      See Abreview

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab63605? Please let us know so that we can cite the reference in this datasheet.

    ab63605 has been referenced in 5 publications.

    • Hiraoka T  et al. Differential roles of uterine epithelial and stromal STAT3 coordinate uterine receptivity and embryo attachment. Sci Rep 10:15523 (2020). PubMed: 32968170
    • Pascual R  et al. The RNA binding protein CPEB2 regulates hormone sensing in mammary gland development and luminal breast cancer. Sci Adv 6:eaax3868 (2020). PubMed: 32440535
    • Yoshida K  et al. Progesterone signaling during pregnancy in the lab opossum, Monodelphis domestica. Theriogenology 136:101-110 (2019). PubMed: 31254723
    • Huang R  et al. Androgen Receptor Expression and Bicalutamide Antagonize Androgen Receptor Inhibit ß-Catenin Transcription Complex in Estrogen Receptor-Negative Breast Cancer. Cell Physiol Biochem 43:2212-2225 (2017). PubMed: 29069648
    • Bashour NM & Wray S Progesterone directly and rapidly inhibits GnRH neuronal activity via progesterone receptor membrane component 1. Endocrinology 153:4457-69 (2012). PubMed: 22822163

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

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    1-2 of 2 Abreviews

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Progesterone Receptor antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA
    Sample
    Mouse Tissue sections (Uterus)
    Specification
    Uterus
    Permeabilization
    Yes - Triton X 0,3%
    Fixative
    Formaldehyde
    Read More

    Dr. Daniel Murta

    Verified customer

    Submitted Sep 10 2014

    Immunocytochemistry abreview for Anti-Progesterone Receptor antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (uterus)
    Specification
    uterus
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.3% Triton-X during blocking step
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Mr. Nicholas Bashour

    Verified customer

    Submitted Feb 17 2011

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