Anti-Progesterone Receptor antibody [Alpha PR6] (ab2765)

Reviews (5)Q&A (7)References (22)

Key features and details

  • Mouse monoclonal [Alpha PR6] to Progesterone Receptor
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Progesterone Receptor antibody [Alpha PR6]
    See all Progesterone Receptor primary antibodies

  • Description

    Mouse monoclonal [Alpha PR6] to Progesterone Receptor

  • Host species

    Mouse

  • Specificity

    Detects the B form of the progesterone receptor (PR). This antibody does not cross-react with estrogen receptor or glucocorticoid receptor.

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Human

  • Immunogen

    Other Immunogen Type corresponding to Chicken Progesterone Receptor. Progesterone receptor purified from chick oviduct cytosol.

Properties

Applications

Our Abpromise guarantee covers the use of ab2765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa.
EMSA Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
    Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
    Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

  • Post-translational
    modifications

    Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
    Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
    Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.

  • Cellular localization

    Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • NR3C3 antibody
    • Nuclear receptor subfamily 3 group C member 3 antibody
    • PGR antibody
    • PR antibody
    • PRA antibody
    • PRB antibody
    • PRGR_HUMAN antibody
    • Progesterone receptor antibody
    • Progestin receptor form A antibody
    • Progestin receptor form B antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)
    Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)

    Immunocytochemistry/ Immunofluorescence analysis of T47D cells untreated (left) or stimulated with 100nm promegestone for 1 hour (right), labeling Progesterone Receptor with ab2765 (green). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA for 15 minutes at room temperature. Cells were stained with Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765) at a dilution of 1/100 for 1 hour at 37C, and then incubated with a Alexa Fluor 488 goat anti-mouse IgG secondary antibody at a dilution of 1/1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye.

  • Western blot - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)
    Western blot - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)
    All lanes : Anti-Progesterone Receptor antibody [Alpha PR6] (ab2765) at 1 µg/ml

    Lane 1 : T47D cell lysate untreated (-)
    Lane 2 : T47D cell lysate stimulated (+) with 100 nm promegestone for 1 hour

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG-HRP at 1/2000 dilution

    Predicted band size: 99 kDa

  • Flow Cytometry - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)
    Flow Cytometry - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)
    Overlay histogram showing T47D cells stained with ab2765 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2765, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

Publishing research using ab2765? Please let us know so that we can cite the reference in this datasheet.

ab2765 has been referenced in 22 publications.

  • Xiao L  et al. Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression. J Cell Physiol N/A:N/A (2019). PubMed: 30671954
  • Park SY  et al. Distribution of and steroid hormone effects on calbindin-D9k in the immature rat brain. Brain Res Bull 152:225-235 (2019). PubMed: 31357009
  • Jin X  et al. ERa is required for suppressing OCT4-induced proliferation of breast cancer cells via DNMT1/ISL1/ERK axis. Cell Prolif 52:e12612 (2019). PubMed: 31012189
  • Xin L  et al. A collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells facilitates endometrial regeneration and restores fertility. Acta Biomater 92:160-171 (2019). PubMed: 31075515
  • Zhang Y  et al. Protective Effect of Taohong Siwu Decoction on Abnormal Uterine Bleeding Induced by Incomplete Medical Abortion in Rats during Early Pregnancy. Chem Pharm Bull (Tokyo) 66:708-713 (2018). PubMed: 29657247
View all Publications for this product

Customer reviews and Q&As

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1-10 of 12 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Sheep Cell (fetal sheep cells)
Permeabilization
No
Specification
fetal sheep cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

Submitted Jan 29 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (sheep uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citric acid,pH 6.0, 0,05% Tween 20
Permeabilization
Yes - Triton-X100 0.2%
Specification
sheep uterus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

Submitted Jan 29 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade

 Poor
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Uterus)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Uterus
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

Submitted Jun 21 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
placenta
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

Submitted Sep 01 2015

Question

Vielen Dank für Ihre schnelle Antwort auf meine Anfrage! Leider ist die vollständige Aminosäuresequenz des Fusionsproteins in der Literatur nicht zu finden.

Der Beschreibung nach handelt es sich um Cre-Rekombinase (AS-Sequenz: http://www.uniprot.org/uniprot/P06956 ?) kombiniert mit einem „nuclear localisation signal“ (Pro-Lys-Lys-Lys-Arg-Lys-Val).

Hinzu kommt eine mutierte Form (hPR891) der „hormone-binding domain“ (HBD; aa 641–891) des humanen Progesteronrezeptors, bei welcher die C-terminalen 42 Aminosäuren fehlen. Diese hätte also vermutlich folgende AS-Sequenz:

FNKVRVVRA LDAVALPQPV GVPNESQALS QRFTFSPGQD IQLIPPLINL LMSIEPDVIY AGHDNTKPDT SSSLLTSLNQ LGERQLLSVV KWSKSLPGFR NLHIDDQITL IQYSWMSLMV FGLGWRSYKH VSGQMLYFAP DLILNEQRMK ESSFYSLCLT MWQIPQEFVK LQVSQEEFLC MKVLLLLNTI PLEGLRSQTQ FEEMRSSYI

Unter diesen Voraussetzungen, wäre es Ihrer Einschätzung nach sicherer einen der vorhandenen Progesteronrezeptor-Antikörper zu verwenden, oder einen Cre-Antikörper? Der Antikörper soll zur immunhistochemischen Färbung von cryofixiertem Gewebe transgener Mäuse verwendet werden.

Haben Sie herzlichen Dank für Ihre Unterstützung!


Mit freundlichen Grüßen

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Answer

Vielen Dank für diese zusätzliche Information.

Leider haben wir keinen Antikörper den ich für die Detektion Ihres rekombinanten Proteins garantieren kann. Ich habe drei Antikörper gefunden die vielleicht für Sie in Frage kommen und die Sie sich genauer anschauen sollten.

1. https://www.abcam.com/cre-recombinase-antibody-723-ab24607.html

Immunogensequenz ist das gesamte native Cre Rekombinase Protein. Die Sequenz sollte daher mit der Sequenz in dem rekombinanten Protein übereinstimmen.

2. https://www.abcam.com/cre-recombinase-antibody-ab137240.html

Bei dem Immunogen handelt es sich um ein rekombinantes full length protein des Bacteriophage P1 Cre recombinase.

3. https://www.abcam.com/progesterone-receptor-antibody-alpha-pr6-ab2765.html

Die Immunogensequenz dieses Antikörpers ist leider nicht näher bestimmt. Der Antikörper wurde gegen das gesamte Hühnchen Progesteron gerichtet hergestellt. Die Hühnchensequenz verglichen mit der zur Verfügung gestellten Progesteronsequenz des rekombinanten Proteins weißt eine Uebereinstimmung von 78% auf. Die Detektion eines Proteins werten wir als wahrscheinlich, wenn eine Übereinstimmung von etwa 85% besteht.



Der ab24607 ist unter diesen drei Möglichkeiten der mit den meisten Referenzen und eingereichten Abreviews und daher am besten charakterisiert. Ab24607 wurde schon für IHC-Fr in Maus verwendet und zwei Abreviews speziell hierfür wurden verfasst.

Ich hoffe ich konnte Ihnen etwas weiter helfen. Es tut mir Leid dass ich Ihnen in diesem Fall kein perfektes Produkt anbieten kann.

Sollten Sie sich dafür entscheiden einen dieser Antikörper zu kaufen, möchte ich Sie noch auf unsere RabMab Aktion aufmerksam machen. Bitte lesen Sie mehr über diese Aktionhttps://www.abcam.com/index.html?pageconfig=resource&rid=15447.

Bitte zögern Sie nicht sich wieder bei mir zu melden, sollten Sie weitere Fragen oder Probleme mit unseren Produkten haben.

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Question

What is the volume in ul for ab2765

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Answer

Thank you for contacting us.

It will be 100ul.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
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Question

storage buffer composition? Ab purified?

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Answer

Yes, the antibody is purified. The solution before lyophilization is 1X PBS with 0.05% sodium azide. I hope this is helpful. Please contact us again if you have any further questions.

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Question

storage buffer composition? Ab purified?

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Answer

Thank you for contacting us. I can confirm that the storage buffer for this antibody is PBS containing 0.05% sodium azide, and that it is purified from mouse ascites. Should I receive any further information from the lab, then I will let you know. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Progesterone Receptor antibody [Alpha PR6]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (mammary carcinoma)
Specification
mammary carcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pressure cooker
Permeabilization
No
Blocking step
BSA as blocking agent for 5 minute(s) · Concentration: 5% · Temperature: RT°C
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孝子 赤松

Verified customer

Submitted Sep 27 2011

Question

BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 217040 DESCRIPTION OF THE PROBLEM Non-specific band.Th strongest band located at 50kDa and second strong band is at 23 KDa. No band show at 99KDa. SAMPLE protein from kidney cortex PRIMARY ANTIBODY overnight at 4C DETECTION METHOD LumiGLO POSITIVE AND NEGATIVE CONTROLS USED No ANTIBODY STORAGE CONDITIONS -20C SAMPLE PREPARATION Homogenization Buffer( 40mM Tris HCl,40mM Tris Base) with 1:100 Protease inhibitor Cocktail Set III(Calbiochem#539134). at 95C for 5min and then on ice AMOUNT OF PROTEIN LOADED 23ug ELECTROPHORESIS/GEL CONDITIONS Reducing ,15% gel TRANSFER AND BLOCKING CONDITIONS taransfer at 0.5 A for 1 hour. Block 1hour with 5% milk. SECONDARY ANTIBODY 1:10000 for 1 hour HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? No

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Answer

Thank you for your enquiry. I am sorry to hear that you have been experiencing problems with ab2675 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 90 days of purchase), and if it appears that the antibody is at fault, a credit note/refund will be offered. I have looked through your protocols and they seem perfectly fine to me. However, I have a few questions that I hope you can answer so that I can understand the problem better. 1) I might not know much about this field but is the expression of progesterone receptor found to be high in the kidney cortex? I would assume that the bands you observed are non-specific. Please try using a positive control like oviduct of chicks with your samples. You can also refer to the references found on the datasheet of this product for more information. 2) Can you please confirm the species from which you are extracting the kidney cortex protein from? This product has only been in tested in Human, Rabbit, Rat, Mouse, Cow, Guinea Pig and Chicken. 3) What was the amount of primary antibody you used to incubate the blot? We would normally suggest 1:300-1:3000 but it is up to the end user to optimize the results. Also, what was the buffer you used to incubate the primary and secondary antibodies? 4) You can also try using a lower percentage gel, like 8-10%, as the protein size of this antibody is expected to be around 99kDa. 5) You mentioned that you used reduced condition. Can you confirm that you also included SDS and beta-mercaptoethanol in the buffer and at what concentration? If the above suggestions did you help you resolve your problem, please get back to me with an image of your immunoblot and purchase details (lot number, shipping address, etc). Should you require any further information or assistance, please do not hesitate to contact me.

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1-10 of 12 Abreviews or Q&A

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