Anti-Progesterone Receptor antibody [Alpha PR6] (ab2765)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [Alpha PR6] (ab2765)](https://www.abcam.com/ps/products/2/ab2765/Images/ab2765-218988-anti-progesterone-receptor-antibody-alpha-pr6-chip-grade-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [Alpha PR6] (ab2765)")
Key features and details
- Mouse monoclonal [Alpha PR6] to Progesterone Receptor
- Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Progesterone Receptor antibody [Alpha PR6]
See all Progesterone Receptor primary antibodies -
Description
Mouse monoclonal [Alpha PR6] to Progesterone Receptor -
Host species
Mouse -
Specificity
Detects the B form of the progesterone receptor (PR). This antibody does not cross-react with estrogen receptor or glucocorticoid receptor. -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Chicken Progesterone Receptor. Progesterone receptor purified from chick oviduct cytosol.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
Alpha PR6 -
Isotype
IgG2a -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (5) |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa.
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| Flow Cyt |
Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-P
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa. |
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Flow Cyt
Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Post-translational
modificationsPhosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation. -
Cellular localization
Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 5241 Human
- Omim: 607311 Human
- SwissProt: P06401 Human
- Unigene: 32405 Human
- Unigene: 742403 Human
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Alternative names
- NR3C3 antibody
- Nuclear receptor subfamily 3 group C member 3 antibody
- PGR antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [Alpha PR6] (ab2765)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on human uterus tissue. Antigen retrieval was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2765 (1:20) overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.
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![Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)](https://www.abcam.com/ps/products/2/ab2765/Images/ab2765-275863-anti-progesterone-receptor-antibody-alpha-pr6-chip-grade-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)Immunocytochemistry/ Immunofluorescence analysis of T47D cells untreated (left) or stimulated with 100nm promegestone for 1 hour (right), labeling Progesterone Receptor with ab2765 (green). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA for 15 minutes at room temperature. Cells were stained with Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765) at a dilution of 1/100 for 1 hour at 37C, and then incubated with a Alexa Fluor 488 goat anti-mouse IgG secondary antibody at a dilution of 1/1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye.
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![Western blot - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)](https://www.abcam.com/ps/products/2/ab2765/Images/ab2765-275864-anti-progesterone-receptor-antibody-alpha-pr6-chip-grade-western-blot.jpg)
Western blot - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)All lanes : Anti-Progesterone Receptor antibody [Alpha PR6] (ab2765) at 1 µg/ml
Lane 1 : T47D cell lysate untreated (-)
Lane 2 : T47D cell lysate stimulated (+) with 100 nm promegestone for 1 hour
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG-HRP at 1/2000 dilution
Predicted band size: 99 kDa -
![Flow Cytometry - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)](https://www.abcam.com/ps/products/2/ab2765/Images/ab2765-6328-anti-progesterone-receptor-antibody-alpha-pr6-chip-grade-flow-cytometry.jpg)
Flow Cytometry - Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765)Overlay histogram showing T47D cells stained with ab2765 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2765, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab2765 has been referenced in 25 publications.
- Tiemann TT et al. Towards uterus tissue engineering: a comparative study of sheep uterus decellularisation. Mol Hum Reprod 26:167-178 (2020). PubMed: 31980817
- Kim YY et al. Effects of Natural Progesterone and Synthetic Progestin on Germ Layer Gene Expression in a Human Embryoid Body Model. Int J Mol Sci 21:N/A (2020). PubMed: 31991577
- Yao X et al. Unconservative_15_2570409 suppresses progesterone receptor expression in the granulosa cells of Hu sheep. Theriogenology 157:303-313 (2020). PubMed: 32827988
- Xiao L et al. Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression. J Cell Physiol N/A:N/A (2019). PubMed: 30671954
- Park SY et al. Distribution of and steroid hormone effects on calbindin-D9k in the immature rat brain. Brain Res Bull 152:225-235 (2019). PubMed: 31357009