• Product name
    Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade
    See all Progesterone Receptor primary antibodies
  • Description
    Mouse monoclonal [Alpha PR6] to Progesterone Receptor - ChIP Grade
  • Host species
  • Specificity
    Detects the B form of the progesterone receptor (PR). This antibody does not cross-react with estrogen receptor or glucocorticoid receptor.
  • Tested applications
    Suitable for: ICC, IHC-Fr, IP, WB, ChIP, Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Human
  • Immunogen

    Other Immunogen Type corresponding to Chicken Progesterone Receptor. Progesterone receptor purified from chick oviduct cytosol.



Our Abpromise guarantee covers the use of ab2765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-Fr Use a concentration of 20 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa.
ChIP Use at an assay dependent concentration.
EMSA Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


ICC/IF Use at an assay dependent concentration.
IHC-P 1/50.


  • Function
    The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
    Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
    Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
    Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
    Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
  • Cellular localization
    Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • NR3C3 antibody
    • Nuclear receptor subfamily 3 group C member 3 antibody
    • PGR antibody
    • PR antibody
    • PRA antibody
    • PRB antibody
    • PRGR_HUMAN antibody
    • Progesterone receptor antibody
    • Progestin receptor form A antibody
    • Progestin receptor form B antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on human uterus tissue. Antigen retrieval was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2765 (1:20) overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

  • Immunocytochemistry/ Immunofluorescence analysis of T47D cells untreated (left) or stimulated with 100nm promegestone for 1 hour (right), labeling Progesterone Receptor with ab2765 (green). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA for 15 minutes at room temperature. Cells were stained with Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765) at a dilution of 1/100 for 1 hour at 37C, and then incubated with a Alexa Fluor 488 goat anti-mouse IgG secondary antibody at a dilution of 1/1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye.

  • All lanes : Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765) at 1 µg/ml

    Lane 1 : T47D cell lysate untreated (-)
    Lane 2 : T47D cell lysate stimulated (+) with 100 nm promegestone for 1 hour

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat anti-Mouse IgG-HRP at 1/2000 dilution

    Predicted band size: 99 kDa

  • ab2765 staining Progesterone Receptor in Rat mammary tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval by pressure cooker prior to blocking with 5% BSA for 5 minutes at room temperature. The primary antibody was diluted 1/100 in TBST and incubated with the sample for 16 hours at 4°C. A biotin-conjugated goat anti-mouse polyclonal was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing T47D cells stained with ab2765 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2765, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


This product has been referenced in:
  • Xiao L  et al. Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 30671954) »
  • Dökümcü K  et al. miR4673 improves fitness profile of neoplastic cells by induction of autophagy. Cell Death Dis 9:1068 (2018). Read more (PubMed: 30341280) »
See all 18 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Dog Tissue sections (Uterus)
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jun 21 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 01 2015


Vielen Dank für diese zusätzliche Information.

Leider haben wir keinen Antikörper den ich für die Detektion Ihres rekombinanten Proteins garantieren kann. Ich habe drei Antikörper gefunden die vielleicht für Sie in Frage kommen und die Sie sich genauer anschauen sollten.

1. https://www.abcam.com/cre-recombinase-antibody-723-ab24607.html

Immunogensequenz ist das gesamte native Cre Rekombinase Protein. Die Sequenz sollte daher mit der Sequenz in dem rekombinanten Protein übereinstimmen.

2. https://www.abcam.com/cre-recombinase-antibody-ab137240.html

Bei dem Immunogen handelt es sich um ein rekombinantes full length protein des Bacteriophage P1 Cre recombinase.

3. https://www.abcam.com/progesterone-receptor-antibody-alpha-pr6-ab2765.html

Die Immunogensequenz dieses Antikörpers ist leider nicht näher bestimmt. Der Antikörper wurde gegen das gesamte Hühnchen Progesteron gerichtet hergestellt. Die Hühnchensequenz verglichen mit der zur Verfügung gestellten Progesteronsequenz des rekombinanten Proteins weißt eine Uebereinstimmung von 78% auf. Die Detektion eines Proteins werten wir als wahrscheinlich, wenn eine Übereinstimmung von etwa 85% besteht.

Der ab24607 ist unter diesen drei Möglichkeiten der mit den meisten Referenzen und eingereichten Abreviews und daher am besten charakterisiert. Ab24607 wurde schon für IHC-Fr in Maus verwendet und zwei Abreviews speziell hierfür wurden verfasst.

Ich hoffe ich konnte Ihnen etwas weiter helfen. Es tut mir Leid dass ich Ihnen in diesem Fall kein perfektes Produkt anbieten kann.

Sollten Sie sich dafür entscheiden einen dieser Antikörper zu kaufen, möchte ich Sie noch auf unsere RabMab Aktion aufmerksam machen. Bitte lesen Sie mehr über diese Aktionhttps://www.abcam.com/index.html?pageconfig=resource&rid=15447.

Bitte zögern Sie nicht sich wieder bei mir zu melden, sollten Sie weitere Fragen oder Probleme mit unseren Produkten haben.

Read More


Thank you for contacting us.

It will be 100ul.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!

Read More


Yes, the antibody is purified. The solution before lyophilization is 1X PBS with 0.05% sodium azide. I hope this is helpful. Please contact us again if you have any further questions.

Read More


Thank you for contacting us. I can confirm that the storage buffer for this antibody is PBS containing 0.05% sodium azide, and that it is purified from mouse ascites. Should I receive any further information from the lab, then I will let you know. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Rat Tissue sections (mammary carcinoma)
mammary carcinoma
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pressure cooker
Blocking step
BSA as blocking agent for 5 minute(s) · Concentration: 5% · Temperature: RT°C

孝子 赤松

Verified customer

Submitted Sep 27 2011


Thank you for your enquiry. I am sorry to hear that you have been experiencing problems with ab2675 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 90 days of purchase), and if it appears that the antibody is at fault, a credit note/refund will be offered. I have looked through your protocols and they seem perfectly fine to me. However, I have a few questions that I hope you can answer so that I can understand the problem better. 1) I might not know much about this field but is the expression of progesterone receptor found to be high in the kidney cortex? I would assume that the bands you observed are non-specific. Please try using a positive control like oviduct of chicks with your samples. You can also refer to the references found on the datasheet of this product for more information. 2) Can you please confirm the species from which you are extracting the kidney cortex protein from? This product has only been in tested in Human, Rabbit, Rat, Mouse, Cow, Guinea Pig and Chicken. 3) What was the amount of primary antibody you used to incubate the blot? We would normally suggest 1:300-1:3000 but it is up to the end user to optimize the results. Also, what was the buffer you used to incubate the primary and secondary antibodies? 4) You can also try using a lower percentage gel, like 8-10%, as the protein size of this antibody is expected to be around 99kDa. 5) You mentioned that you used reduced condition. Can you confirm that you also included SDS and beta-mercaptoethanol in the buffer and at what concentration? If the above suggestions did you help you resolve your problem, please get back to me with an image of your immunoblot and purchase details (lot number, shipping address, etc). Should you require any further information or assistance, please do not hesitate to contact me.

Read More


This sounds like a classic case of antibody storage condition problems. The antibody may be binding to the plastic tube as a result of the aliquot size being too small (i.e. less then 10ul). I would appreciate it if you could please tell me the amount they placed in each tube. Another possible reason for this is the antibody may become unstable as a result of no extra protein being added to the tube such as 5% BSA as a cryopretectant. If you would like, we would be more then happy to send a replacement to see how that works.

Read More


I have just contacted the originator of this product. I would like to confirm that the there should always be extra protein in the tube so that it will help stabilize the antibody, especially for a monoclonal antibody. If you are going to use the antibody within a relatively short period of time (i.e a week) I would suggest keeping it at 4 degrees. Since you had a problem with the first vial, we were going to offer you a replacement. However, you have already placed a second order - 1 vial of 2765. Therefore this second vial would be free of charge, you do not need to pay for it. Please do let me know how you are getting on with the second vial. Good luck!

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up