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    progesterone-receptor-antibody-c-terminal-ab191138.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-Progesterone Receptor antibody - C-terminal (ab191138)

  • Datasheet
Reviews (6) Submit a question References (1)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody - C-terminal (ab191138)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody - C-terminal (ab191138)
  • Western blot - Anti-Progesterone Receptor antibody - C-terminal (ab191138)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody - C-terminal (ab191138)

Key features and details

  • Rabbit polyclonal to Progesterone Receptor - C-terminal
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Progesterone Receptor antibody [YR85] (ab32085)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Progesterone Receptor antibody - C-terminal
    See all Progesterone Receptor primary antibodies
  • Description

    Rabbit polyclonal to Progesterone Receptor - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Chicken, Dog, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human Progesterone Receptor aa 550 to the C-terminus (C terminal). (E. coli-derived).
    Database link: P06401

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa, MCF7 and SKOV Whole Cell Lysates; Rat and Mouse Brain Tissue Lysates; Human Lung, Mammary and Intestinal Cancer Tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.025% Sodium azide
    Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Progesterone
    • Neuroscience
    • Endocrine system
    • Gonadotrophic axis
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Progesterone
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Progesterone
    • Cancer
    • Tumor biomarkers
    • Receptors
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human Progesterone Receptor protein (ab159105)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab191138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (6)
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 99 kDa.
Notes
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 99 kDa.

Target

  • Function

    The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
    Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
    Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications

    Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
    Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
    Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
  • Cellular localization

    Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
  • Target information above from: UniProt accession P06401 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 396198 Chicken
    • Entrez Gene: 403621 Dog
    • Entrez Gene: 5241 Human
    • Entrez Gene: 18667 Mouse
    • Entrez Gene: 100009094 Rabbit
    • Omim: 607311 Human
    • SwissProt: A7X8B3 Chimpanzee
    • SwissProt: A7X8B7 Gorilla
    • SwissProt: P06401 Human
    • SwissProt: Q00175 Mouse
    • Unigene: 32405 Human
    • Unigene: 742403 Human
    • Unigene: 12798 Mouse
    • Unigene: 437703 Mouse
    see all
  • Alternative names

    • NR3C3 antibody
    • Nuclear receptor subfamily 3 group C member 3 antibody
    • PGR antibody
    • PR antibody
    • PRA antibody
    • PRB antibody
    • PRGR_HUMAN antibody
    • Progesterone receptor antibody
    • Progestin receptor form A antibody
    • Progestin receptor form B antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody - C-terminal (ab191138)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody - C-terminal (ab191138)

    Immunohistochemical analysis of paraffin-embedded Human Intestinal Cancer Tissue labeling Progesterone Receptor with ab191138 at 1 µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody - C-terminal (ab191138)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody - C-terminal (ab191138)

    Immunohistochemical analysis of paraffin-embedded Human Mammary Cancer Tissue labeling Progesterone Receptor with ab191138 at 1 µg/ml.

  • Western blot - Anti-Progesterone Receptor antibody - C-terminal (ab191138)
    Western blot - Anti-Progesterone Receptor antibody - C-terminal (ab191138)
    Anti-Progesterone Receptor antibody - C-terminal (ab191138) at 0.1 µg/ml + Human T-47D whole cell lysates. at 50 µg with 5% non-fat milk/ TBS for 1.5 hours at room temperature.

    Secondary
    Goat anti-Rabbit IgG-HRP for 1.5 hours at room temperature at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 99 kDa
    Additional bands at: 90 kDa (possible isoform), 99 kDa (possible isoform)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody - C-terminal (ab191138)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody - C-terminal (ab191138)

    Immunohistochemical analysis of paraffin-embedded Human Lung Cancer Tissue labeling Progesterone Receptor with ab191138 at 1 µg/ml.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (1)

Publishing research using ab191138? Please let us know so that we can cite the reference in this datasheet.

ab191138 has been referenced in 1 publication.

  • Quast A  et al. Donor variation and sex hormone receptors in periodontal ligament cells. Arch Oral Biol 122:105026 (2021). PubMed: 33348207

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Progesterone Receptor antibody - C-terminal

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: high Target retrival solution
Permeabilization
No
Specification
uterus
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
Fixative
Formaldehyde
Read More

FRAU. Ines Kleiber-Schaaf

Verified customer

Submitted Jul 11 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Progesterone Receptor antibody - C-terminal

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (mammary gland tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
Yes - 3% H2O2
Specification
mammary gland tumor
Fixative
Formaldehyde
Read More

Ms. Hyojeong Han

Verified customer

Submitted Feb 12 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Progesterone Receptor antibody - C-terminal

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate ph 6,0
Permeabilization
No
Specification
uterus
Blocking step
Background Block (CELL MARQUE) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Prof. ALESSANDRA MEDEIROS

Verified customer

Submitted Oct 15 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Progesterone Receptor antibody - C-terminal

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
uterus
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted May 23 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Progesterone Receptor antibody - C-terminal

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
testis
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Aug 13 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Progesterone Receptor antibody - C-terminal

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
placenta
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jul 17 2015

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