Product nameAnti-Progesterone Receptor antibody - C-terminal
See all Progesterone Receptor primary antibodies
DescriptionRabbit polyclonal to Progesterone Receptor - C-terminal
SpecificityNo cross reactivity with other proteins.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Chicken, Dog, Chimpanzee, Gorilla, Orangutan
Recombinant fragment corresponding to Human Progesterone Receptor aa 595-933 (C terminal). (E. coli-derived).
MEGQHNYLCAGRNDCIVDKIRRKNCPACRLRKCCQAGMVLGGRKFKKFNK VRVVRALDAVALPQPVGVPNESQALSQRFTFSPGQDIQLIPPLINLLMSI EPDVIYAGHDNTKPDTSSSLLTSLNQLGERQLLSVVKWSKSLPGFRNLHI DDQITLIQYSWMSLMVFGLGWRSYKHVSGQMLYFAPDLILNEQRMKESSF YSLCLTMWQIPQEFVKLQVSQEEFLCMKVLLLLNTIPLEGLRSQTQFEEM RSSYIRELIKAIGLRQKGVVSSSQRFYQLTKLLDNLHDLVKQLHLYCLNT FIQSRALSVEFPEMMSEVIAAQLPKILAGMVKPLLFHKK
Database link: P06401
- HeLa, MCF7 and SKOV Whole Cell Lysates; Rat and Mouse Brain Tissue Lysates; Human Lung, Mammary and Intestinal Cancer Tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.025% Sodium azide
Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 99 kDa.|
FunctionThe steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
modificationsPhosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
Cellular localizationNucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
- Information by UniProt
- NR3C3 antibody
- Nuclear receptor subfamily 3 group C member 3 antibody
- PGR antibody
Immunohistochemical analysis of paraffin-embedded Human Intestinal Cancer Tissue labeling Progesterone Receptor with ab191138 at 1 µg/ml.
Immunohistochemical analysis of paraffin-embedded Human Mammary Cancer Tissue labeling Progesterone Receptor with ab191138 at 1 µg/ml.
Anti-Progesterone Receptor antibody - C-terminal (ab191138) at 0.1 µg/ml + Human T-47D whole cell lysates. at 50 µg with 5% non-fat milk/ TBS for 1.5 hours at room temperature.
Goat anti-Rabbit IgG-HRP for 1.5 hours at room temperature at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 99 kDa
Additional bands at: 90 kDa (possible isoform), 99 kDa (possible isoform)
Immunohistochemical analysis of paraffin-embedded Human Lung Cancer Tissue labeling Progesterone Receptor with ab191138 at 1 µg/ml.
ab191138 has not yet been referenced specifically in any publications.