Recombinant Anti-Progesterone Receptor antibody [SP2] (ab16661)
Rabbit monoclonal Progesterone Receptor antibody [SP2]. Validated in WB, IHC, Flow Cyt, ICC/IF and tested in Rat, Human. Cited in 11 publication(s). Independently reviewed in 2 review(s).
- Datasheet
- References (24)
- Protocols
Overview
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Product name
Anti-Progesterone Receptor antibody [SP2]
See all Progesterone Receptor primary antibodies -
Description
Rabbit monoclonal [SP2] to Progesterone Receptor -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Rabbit
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Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
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Positive control
- Breast carcinomas
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General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com
This product was switched from a hybridoma to recombinant production method on 13th November 2019.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP2 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab16661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| WB | 1/200. Predicted molecular weight: 99,120 kDa. | |
| IHC-P | 1/400. Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
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| Flow Cyt | 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Post-translational
modificationsPhosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation. -
Cellular localization
Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 5241 Human
- Entrez Gene: 100009094 Rabbit
- Entrez Gene: 25154 Rat
- Omim: 607311 Human
- SwissProt: P06401 Human
- SwissProt: Q63449 Rat
- Unigene: 32405 Human
- Unigene: 742403 Human
see all -
Alternative names
- NR3C3 antibody
- Nuclear receptor subfamily 3 group C member 3 antibody
- PGR antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)](https://www.abcam.com/ps/products/16/ab16661/Images/ab16661-333826-anti-progesterone-receptor-antibody-sp2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)Immunocytochemistry/ Immunofluorescence analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab16661 at 1/100 (2.28 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)](https://www.abcam.com/ps/products/16/ab16661/Images/ab16661-333798-anti-progesterone-receptor-antibody-sp2-flow-cytometry.jpg)
Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)Flow Cytometry analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab16661 at 1/220 dilution (1.04 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)](https://www.abcam.com/ps/products/16/ab16661/Images/ab16661-249496-anti-progesterone-receptor-antibody-sp2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)Immunohistochemistry analysis of human breast carcinoma tissue labelling SP2 with ab16661.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)](https://www.abcam.com/ps/products/16/ab16661/Images/ab16661-25515-anti-progesterone-receptor-antibody-sp2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)This image is courtesy of an anonymous Abreviewab16661 staining rat ovary tissue sections by IHC-P. Sections were fixed in 10% buffered formalin and subjected to heat mediated antigen retrieval in 10mM citrate buffer pH 6.0 prior to blocking with 5% serum for 20 minutes at 20°C. The primary antibody was diluted 1/50 in TBS (with Tween) and incubated with the sample for 16 hours at 4°C. A HRP conjugated goat anti-rabbit was used as the secondary antibody.
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![Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)](https://www.abcam.com/ps/products/16/ab16661/Images/ab16661-25514-anti-progesterone-receptor-antibody-sp2-flow-cytometry.jpg)
Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)Overlay histogram showing T47D cells stained with ab16661 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16661, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
References
This product has been referenced in:
- Liu Y et al. Efficacy and safety of TE/TEC/intensive paclitaxel neoadjuvant chemotherapy for the treatment of breast cancer. Oncol Lett 17:907-912 (2019). IHC-P, IHC ; Human . Read more (PubMed: 30655846) »
- Sun L et al. The Modified Bushen Antai Recipe Upregulates Estrogen and Progesterone Receptors at the Maternal-Fetal Interface in Pregnant Rats with Mifepristone-Induced Pregnancy Loss. Evid Based Complement Alternat Med 2019:8312020 (2019). IHC-P, IHC ; Rat . Read more (PubMed: 30792746) »
