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  1. Link

    progesterone-receptor-antibody-sp2-ab16661.pdf

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RecombinantRabMAb

Recombinant Anti-Progesterone Receptor antibody [SP2] (ab16661)

  • Datasheet
  • SDS
Reviews (4)Q&A (5)References (32)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)
  • Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)
  • Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)
  • Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)
  • Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)
  • Anti-Progesterone Receptor antibody [SP2] (ab16661)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP2] to Progesterone Receptor
  • Suitable for: ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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AMG 9810, TRPV1 receptor antagonist (ab145874)
Protein
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Recombinant Human Progesterone Receptor protein (ab159105)
Primary
Product image
Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)

View more associated products

Overview

  • Product name

    Anti-Progesterone Receptor antibody [SP2]
    See all Progesterone Receptor primary antibodies
  • Description

    Rabbit monoclonal [SP2] to Progesterone Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Rabbit
  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • Breast carcinomas
  • General notes

    This product was switched from a hybridoma to recombinant production method on 13th November 2019.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    SP2
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Progesterone
    • Neuroscience
    • Endocrine system
    • Gonadotrophic axis
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Progesterone
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Progesterone
    • Cancer
    • Tumor biomarkers
    • Receptors
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Progesterone Receptor antibody [SP2] - BSA and Azide free (ab239793)
    • Alexa Fluor® 488 Anti-Progesterone Receptor antibody [SP2] (ab267523)
    • Alexa Fluor® 647 Anti-Progesterone Receptor antibody [SP2] (ab267524)
    • Anti-Progesterone Receptor antibody [SP2], prediluted (ab27596)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Progesterone Receptor protein (ab159105)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
IHC-P (3)
1/400.

Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Flow Cyt
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Notes
ICC/IF
1/100.
IHC-P
1/400.

Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Flow Cyt
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
    Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
    Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications

    Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
    Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
    Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
  • Cellular localization

    Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
  • Target information above from: UniProt accession P06401 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5241 Human
    • Entrez Gene: 100009094 Rabbit
    • Entrez Gene: 25154 Rat
    • Omim: 607311 Human
    • SwissProt: P06401 Human
    • SwissProt: Q63449 Rat
    • Unigene: 32405 Human
    • Unigene: 742403 Human
    • Unigene: 10303 Rat
    see all
  • Alternative names

    • NR3C3 antibody
    • Nuclear receptor subfamily 3 group C member 3 antibody
    • PGR antibody
    • PR antibody
    • PRA antibody
    • PRB antibody
    • PRGR_HUMAN antibody
    • Progesterone receptor antibody
    • Progestin receptor form A antibody
    • Progestin receptor form B antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)

    Immunohistochemistry analysis of human breast carcinoma tissue labelling SP2 with ab16661.

    This image was generated from the hybridoma version.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)
    Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)

    Immunocytochemistry/ Immunofluorescence analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab16661 at 1/100 (2.28 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This image was generated from the hybridoma version.

     

  • Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)
    Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)

    Flow Cytometry analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab16661 at 1/220 dilution (1.04 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.

    This image was generated from the hybridoma version.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)This image is courtesy of an anonymous Abreview

    ab16661 staining rat ovary tissue sections by IHC-P.  Sections were fixed in 10% buffered formalin and subjected to heat mediated antigen retrieval in 10mM citrate buffer pH 6.0 prior to blocking with 5% serum for 20 minutes at 20°C.  The primary antibody was diluted 1/50 in TBS (with Tween) and incubated with the sample for 16 hours at 4°C.  A HRP conjugated goat anti-rabbit was used as the secondary antibody.

    This image was generated from the hybridoma version.

     

    See Abreview

  • Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)
    Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)

    Overlay histogram showing T47D cells stained with ab16661 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16661, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated from the hybridoma version.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)
    Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)Ding L, et al. (2019), PLOS Genetics, 15(3), e1008002. Fig 3D, DOI: 10.1371/journal.pgen.1008002. Reproduced under the Creative Commons licence. https://creativecommons.org/licenses/by/4.0/

    Rat mammary epithelial cells stained for Pr (pink) using ab16661 at 1/100 dilution in ICC/IF. 

  • Anti-Progesterone Receptor antibody [SP2] (ab16661)
    Anti-Progesterone Receptor antibody [SP2] (ab16661)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (32)

Publishing research using ab16661? Please let us know so that we can cite the reference in this datasheet.

ab16661 has been referenced in 32 publications.

  • Yokomizo R  et al. Endometrial regeneration with endometrial epithelium: homologous orchestration with endometrial stroma as a feeder. Stem Cell Res Ther 12:130 (2021). PubMed: 33579355
  • Feng Y  et al. Inhibition of Fibroblast Activation in Uterine Leiomyoma by Components of Rhizoma Curcumae and Rhizoma Sparganii. Front Public Health 9:650022 (2021). PubMed: 33732680
  • Gao J  et al. Risk factors predicting the occurrence of metachronous ovarian metastasis of gastric cancer. Ann Transl Med 9:1049 (2021). PubMed: 34422961
  • Sun C  et al. Comparison between core needle biopsy and excisional biopsy for breast neoplasm. Medicine (Baltimore) 100:e26970 (2021). PubMed: 34449464
  • Meyer AE  et al. Loss of Fbxw7 triggers mammary tumorigenesis associated with E2F/c-Myc activation and Trp53 mutation. Neoplasia 22:644-658 (2020). PubMed: 33070870
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-9 of 9 Abreviews or Q&A

Western blot abreview for Anti-Progesterone Receptor antibody [SP2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U937 cells)
Gel Running Conditions
Reduced Denaturing (4-12% Tris-Bis gel)
Loading amount
40 µg
Specification
U937 cells
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Mar 10 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Progesterone Receptor antibody [SP2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (breast cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: BOND ER2
Permeabilization
No
Specification
breast cancer
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Dec 13 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Progesterone Receptor antibody [SP2]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 20% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9
Sample
Human Tissue sections (Breast carcinoma)
Specification
Breast carcinoma
Permeabilization
No
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jul 15 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Progesterone Receptor antibody [SP2]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat ovary, uterus)
Specification
Rat ovary, uterus
Fixative
10% buffered formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Nov 27 2007

Question

I recently purchased an antibody from yourselves – Progesterone receptor, ab16661, lot: GR52327-3.
Please would you be able to tell me the protein concentration of this antibody.
Many Thanks

Read More

Abcam community

Verified customer

Asked on Feb 29 2012

Answer

Thank you for your enquiry.
I can confirm that ab16661 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.
I can confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml
I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

Abcam Scientific Support

Answered on Feb 29 2012

Question

Hi,

I recently purchased an antibody from yourselves – Progesterone receptor, ab16661, lot: GR52327-3.

Please would you be able to tell me the protein concentration of this antibody.

Many Thanks

Read More

Abcam community

Verified customer

Asked on Feb 29 2012

Answer

Thank you for your enquiry.

I can confirm that ab16661 Progesterone Receptor antibody SP2 is sold as tissue culture supernatant. TC supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

Abcam Scientific Support

Answered on Feb 29 2012

Question

Dear Sir/Madam Please kindly recommend the abcam slide control for IHC-P application for this antibodies. 1. Anti-ErbB 2 antibody (ab2428) 2. Anti-Progesterone Receptor antibody [SP2] (ab16661) 3. Anti-Estrogen Receptor antibody [SP1] (ab16660) 4. Anti-Myeloperoxidase antibody (ab45977) 5. Anti-TdT antibody (ab85148) 6. Anti-TMEM16A antibody (ab53212) 7. Anti-NSE antibody [EPR3377] (ab79757) 8. Anti-CD23 antibody [EPR3617(IHC)] (ab92495) 9. Anti-CD10 antibody [56C6] (ab951) 10. Anti-Melanoma antibody [HMB45 + DT101 + BC199] (ab732) 11. Anti-CD79a antibody [HM47/A9] (ab3121) 12. Anti-CD45RO antibody [UCHL-1] (ab86080) 13. Anti-CD45 antibody (ab10559) 14. Anti-CD30 antibody [CON6D/ C2] - BSA and Azide free (ab76518) 15. Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)] (ab115993) Best regards

Read More

Abcam community

Verified customer

Asked on Jan 09 2012

Answer

Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Human breast cancer 2.Anti-Progesterone Receptor antibody [SP2] (ab16661) Human breast carcinoma 3.Anti-Estrogen Receptor antibody [SP1] (ab16660) Human breast carcinoma 4.Anti-Myeloperoxidase antibody (ab45977) Human spleen tissue 5.Anti-TdT antibody (ab85148) Human testis 6.Anti-TMEM16A antibody (ab53212) Human liver 7.Anti-NSE antibody [EPR3377] (ab79757) Human brain tissue 8.Anti-CD23 antibody [EPR3617(IHC)] (ab92495) Human tonsil 9.Anti-CD10 antibody [56C6] (ab951) Lymph node tissue or human breast microepithelium 10.Anti-Melanoma antibody [HMB45 + DT101 + BC199] (ab732) Murine melanoma metastasis tissue 11.Anti-CD79a antibody [HM47/A9] (ab3121) Human Lymphoid tissue, tonsil 12.Anti-CD45RO antibody [UCHL-1] (ab86080) Human Tonsil, Thymus 13.Anti-CD45 antibody (ab10559) Human Tonsil tissue 14.Anti-CD30 antibody [CON6D/ C2] - BSA and Azide free (ab76518) Human Hodgekins lymphoma tissue 15.Anti-CD15 antibody [(Workshop IV) (BRA-4F1) our designation 238)] (ab115993) Human Hodgekins lymphoma tissue Human spleen I am providing a list ofslides that we sell which I hope will be helpful to you. I can recommend to review the online datasheet for more details. Human brain: ab4305Brain (human): hippocampus normal tissue slides https://www.abcam.com/Brain-human-hippocampus-normal-tissue-slides-ab4305.html (or use the following: https://www.abcam.com/Brain-human-hippocampus-normal-tissue-slides-ab4305.html). Human lymph node: ab4350Lymph node (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Lymph-node-human-normal-tissue-slides-ab4350.html (or use the following: https://www.abcam.com/Lymph-node-human-normal-tissue-slides-ab4350.html). Human testis: ab4373Testis (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Testis-human-normal-tissue-slides-ab4373.html (or use the following: https://www.abcam.com/Testis-human-normal-tissue-slides-ab4373.html). Human liver: ab4348Liver (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Liver-human-normal-tissue-slides-ab4348.html (or use the following: https://www.abcam.com/Liver-human-normal-tissue-slides-ab4348.html). Human spleen: ab4372Spleen (human) normal tissue slides IHC-P, ISH https://www.abcam.com/Spleen-human-normal-tissue-slides-ab4372.html (or use the following: https://www.abcam.com/Spleen-human-normal-tissue-slides-ab4372.html). I am sorry we do not have slides forthe following: Human tonsil Hodgekins lymphoma tissue Mouse melenoma tissue I hope this will be helpful to you. If you have any further questions,please do not hesitate to contact us.

Read More

Abcam Scientific Support

Answered on Jan 09 2012

Question

 I want to konw if ab16661 (Rabbit monoclonal [SP2] to Progesterone Receptor) can be used for IHC of rat ovary tissue's Progesterone Receptor. I want a firm answer. Thank you! Best wishes!               

Read More

Abcam community

Verified customer

Asked on Dec 16 2011

Answer

Thank you for contacting us. This antibody has been tested on human breast carcinoma tissue sections in our own lab and as you can see from image it produced best results. One of the customers has used this antibody on Rat ovary tissue; as you can see from Abreview this also produced good result based on the target localization however we do not know why customer has given three stars to the Abreview. It is actually a good image. I can confirm that the antibody can be used for staining of target on Rat sections. In case the product does not work after applying troubleshooting steps; full amount can be refunded. Please contact our distributor in China for full terms and conditions.    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Check the following compatible products: ab6012       Goat F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin), pre-adsorbed ab6013       Goat F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed ab6015      Goat F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (AP), pre-adsorbed ab6021      Goat F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - Fc (Biotin) ab6023      Goat F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - Fc (HRP) ab6024      Goat F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - Fc (AP) ab96895    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed ab96896    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed For complete please visit website at www.abcam.com

Read More

Abcam Scientific Support

Answered on Dec 16 2011

Question

I want to konw if ab16661 (Rabbit monoclonal [SP2] to Progesterone Receptor) can be used for IHC of rat ovary tissue's Progesterone Receptor. I want a firm answer. Thank you!  

Read More

Abcam community

Verified customer

Asked on Dec 15 2011

Answer

Thank you for contacting Abcam. Under our Abpromise we guarantee that ab16661 will work on rat tissue in IHC-P. As long as the protein is expressed in the ovarian tissues of the rat then this antibody will be able to detect it. If there is anything else I can help you with, please let me kn

Read More

Abcam Scientific Support

Answered on Dec 15 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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