Recombinant Anti-Progesterone Receptor antibody [SP2] (ab16661)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)](https://www.abcam.com/ps/products/16/ab16661/Images/ab16661-249496-anti-progesterone-receptor-antibody-sp2-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP2] to Progesterone Receptor
- Suitable for: ICC/IF, IHC-P, Flow Cyt
- Reacts with: Rat, Human
Overview
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Product name
Anti-Progesterone Receptor antibody [SP2]
See all Progesterone Receptor primary antibodies -
Description
Rabbit monoclonal [SP2] to Progesterone Receptor -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Rabbit
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Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
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Positive control
- Breast carcinomas
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General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com
This product was switched from a hybridoma to recombinant production method on 13th November 2019.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP2 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab16661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| IHC-P | 1/400. Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
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| Flow Cyt | 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Post-translational
modificationsPhosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation. -
Cellular localization
Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 5241 Human
- Entrez Gene: 100009094 Rabbit
- Entrez Gene: 25154 Rat
- Omim: 607311 Human
- SwissProt: P06401 Human
- SwissProt: Q63449 Rat
- Unigene: 32405 Human
- Unigene: 742403 Human
see all -
Alternative names
- NR3C3 antibody
- Nuclear receptor subfamily 3 group C member 3 antibody
- PGR antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)
Immunohistochemistry analysis of human breast carcinoma tissue labelling SP2 with ab16661.
This image was generated from the hybridoma version.
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![Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)](https://www.abcam.com/ps/products/16/ab16661/Images/ab16661-333826-anti-progesterone-receptor-antibody-sp2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)Immunocytochemistry/ Immunofluorescence analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab16661 at 1/100 (2.28 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This image was generated from the hybridoma version.
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![Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)](https://www.abcam.com/ps/products/16/ab16661/Images/ab16661-333798-anti-progesterone-receptor-antibody-sp2-flow-cytometry.jpg)
Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)Flow Cytometry analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab16661 at 1/220 dilution (1.04 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
This image was generated from the hybridoma version.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)](https://www.abcam.com/ps/products/16/ab16661/Images/ab16661-25515-anti-progesterone-receptor-antibody-sp2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)This image is courtesy of an anonymous Abreviewab16661 staining rat ovary tissue sections by IHC-P. Sections were fixed in 10% buffered formalin and subjected to heat mediated antigen retrieval in 10mM citrate buffer pH 6.0 prior to blocking with 5% serum for 20 minutes at 20°C. The primary antibody was diluted 1/50 in TBS (with Tween) and incubated with the sample for 16 hours at 4°C. A HRP conjugated goat anti-rabbit was used as the secondary antibody.
This image was generated from the hybridoma version.
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![Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)](https://www.abcam.com/ps/products/16/ab16661/Images/ab16661-25514-anti-progesterone-receptor-antibody-sp2-flow-cytometry.jpg)
Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)Overlay histogram showing T47D cells stained with ab16661 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16661, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated from the hybridoma version.
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![Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)](https://www.abcam.com/ps/products/16/ab16661/Images/ab16661-395279-AntiProgesterone-Receptor-antibody-SP2-immunofluorescence-mammary-glands.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)Ding L, et al. (2019), PLOS Genetics, 15(3), e1008002. Fig 3D, DOI: 10.1371/journal.pgen.1008002. Reproduced under the Creative Commons licence. https://creativecommons.org/licenses/by/4.0/Rat mammary epithelial cells stained for Pr (pink) using ab16661 at 1/100 dilution in ICC/IF.
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![Anti-Progesterone Receptor antibody [SP2] (ab16661)](https://www.abcam.com/ps/products/16/ab16661/Images/ab16661-10-benefits-of-recombinant-antibodies.png)
Anti-Progesterone Receptor antibody [SP2] (ab16661)
Protocols
References (25)
ab16661 has been referenced in 25 publications.
- Liu Y et al. Efficacy and safety of TE/TEC/intensive paclitaxel neoadjuvant chemotherapy for the treatment of breast cancer. Oncol Lett 17:907-912 (2019). IHC-P, IHC ; Human . PubMed: 30655846
- Sun L et al. The Modified Bushen Antai Recipe Upregulates Estrogen and Progesterone Receptors at the Maternal-Fetal Interface in Pregnant Rats with Mifepristone-Induced Pregnancy Loss. Evid Based Complement Alternat Med 2019:8312020 (2019). IHC-P, IHC ; Rat . PubMed: 30792746
- Ding L et al. Deletion of Cdkn1b in ACI rats leads to increased proliferation and pregnancy-associated changes in the mammary gland due to perturbed systemic endocrine environment. PLoS Genet 15:e1008002 (2019). IHC ; Rat . PubMed: 30893315
- Crobeddu B et al. Di(2-ethylhexyl) phthalate (DEHP) increases proliferation of epithelial breast cancer cells through progesterone receptor dysregulation. Environ Res 173:165-173 (2019). PubMed: 30909102
- Wright KD et al. The p52 isoform of SHC1 is a key driver of breast cancer initiation. Breast Cancer Res 21:74 (2019). IHC . PubMed: 31202267
