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Host species: Goat
Conjugation: Horse Radish Peroxidase
Other pretreatments were also tested eg 100C for 10 minutes, using either 10mM citrate (pH 6.0) or 1mM EDTA buffer (pH 8.0). These produced positive, but less intense, nuclear staining.
This technique produced a similar staining pattern and intensity when the primary antibody was applied at 1:50 for 2 hours at room temperature.
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Submitted Nov 27 2007