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    progesterone-receptor-antibody-sp2-prediluted-ab27596.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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RabMAb

Anti-Progesterone Receptor antibody [SP2], prediluted (ab27596)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (4)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2], prediluted (ab27596)

    Key features and details

    • Rabbit monoclonal [SP2] to Progesterone Receptor, prediluted
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-Progesterone Receptor antibody [SP2], prediluted
      See all Progesterone Receptor primary antibodies
    • Description

      Rabbit monoclonal [SP2] to Progesterone Receptor, prediluted
    • Host species

      Rabbit
    • Specificity

      ab27596 recognises progesterone receptor (PgR).
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant fragment within Human Progesterone Receptor aa 400-550. The exact sequence is proprietary.
      Database link: P06401

    • Positive control

      • Breast carcinomas
    • General notes

      This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.60
      Preservative: 0.1% Sodium azide
      Constituents: Tissue culture supernatant, Tris buffered saline, 1% BSA
    • Concentration information loading...
    • Purity

      Tissue culture supernatant
    • Clonality

      Monoclonal
    • Clone number

      SP2
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Zinc Finger
      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • Nuclear Hormone Receptors
      • Progesterone
      • Neuroscience
      • Endocrine system
      • Gonadotrophic axis
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • Nuclear Receptors
      • Progesterone
      • Cancer
      • Signal transduction
      • Nuclear signaling
      • Nuclear hormone receptors
      • Progesterone
      • Cancer
      • Tumor biomarkers
      • Receptors
      • Neuroscience
      • Development
      • Neuroscience
      • Processes

    Associated products

    • Alternative Versions

      • Anti-Progesterone Receptor antibody [SP2] (ab16661)
      • Anti-Progesterone Receptor antibody [SP2] - BSA and Azide free (ab239793)
      • Alexa Fluor® 647 Anti-Progesterone Receptor antibody [SP2] (ab267524)
    • Isotype control

      • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Recombinant Protein

      • Recombinant Human Progesterone Receptor protein (ab159105)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab27596 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Notes
    Application notes
    IHC-P: Ready to use for 10 minutes at room temperature. Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Target

    • Function

      The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
      Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
      Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Domain

      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • Post-translational
      modifications

      Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
      Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
      Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
      Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
    • Cellular localization

      Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
    • Target information above from: UniProt accession P06401 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5241 Human
      • Omim: 607311 Human
      • SwissProt: P06401 Human
      • Unigene: 32405 Human
      • Unigene: 742403 Human
      • Alternative names

        • NR3C3 antibody
        • Nuclear receptor subfamily 3 group C member 3 antibody
        • PGR antibody
        • PR antibody
        • PRA antibody
        • PRB antibody
        • PRGR_HUMAN antibody
        • Progesterone receptor antibody
        • Progestin receptor form A antibody
        • Progestin receptor form B antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2], prediluted (ab27596)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2], prediluted (ab27596)
        Staining of human breast carcinoma using ab27596

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (4)

      Publishing research using ab27596? Please let us know so that we can cite the reference in this datasheet.

      ab27596 has been referenced in 4 publications.

      • Onder S  et al. Loss of ARID1A expression is associated with poor prognosis in invasive micropapillary carcinomas of the breast: A clinicopathologic and immunohistochemical study with long-term survival analysis. Breast J 23:638-646 (2017). PubMed: 28543794
      • Tian M & Schiemann WP TGF-ß Stimulation of EMT Programs Elicits Non-genomic ER-a Activity and Anti-estrogen Resistance in Breast Cancer Cells. J Cancer Metastasis Treat 3:150-160 (2017). PubMed: 28955730
      • Park SY  et al. Does background parenchymal enhancement on MRI affect the rate of positive resection margin in breast cancer patients? Br J Radiol 88:20140638 (2015). PubMed: 25429418
      • Sertedaki A  et al. Long-term clinical data and molecular defects in the STAR gene in five Greek patients. Eur J Endocrinol 168:351-9 (2013). PubMed: 23211570

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Dear All,



      Please let us know about the following that many slides should have been available for the concentrated antibodies after dilution and how many slides can be prepeared with the prediluted slides. Also let us know the about the deteation kit and DAB ab64264 and



      ab3223

      ab63340

      ab74179

      ab8105

      ab27595 (pre-diluted)

      ab27596 (pre-diluted)

      ab15467 (pre-diluted)


      ab64264 -sufficient for how many slides?

      Awaiting your early response.

      Sincere regards,

      Read More

      Abcam community

      Verified customer

      Asked on Jan 26 2012

      Answer

      Thank you for your inquiry.

      Since the working dilutions of antibodies are not a fixed value but have to optimised by the endusers themselves according to experimental condition, I cannot give you absolute numbers. Even when the pre-diluted antibodies are used neat there might be reasons to use 50ul per slide or maybe even 100ul per slide.

      These conditions include: size of the sample, tissue type, lab experience, diluent buffer, species, antigen retrieval, incubation time, etc.

      For the products you mentioned I can give a estimate for the pre-diluted antibodies of ca 140- 280slides, for the other antibodies 500 - 100 slides and for the detection kit 150 - 300 slides.

      I hope this information is helpful.

      Read More

      Abcam Scientific Support

      Answered on Jan 26 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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