For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Please let us know about the following that many slides should have been available for the concentrated antibodies after dilution and how many slides can be prepeared with the prediluted slides. Also let us know the about the deteation kit and DAB ab64264 and
ab64264 -sufficient for how many slides?
Awaiting your early response.
Asked on Jan 26 2012
Thank you for your inquiry.
Since the working dilutions of antibodies are not a fixed value but have to optimised by the endusers themselves according to experimental condition, I cannot give you absolute numbers. Even when the pre-diluted antibodies are used neat there might be reasons to use 50ul per slide or maybe even 100ul per slide.
These conditions include: size of the sample, tissue type, lab experience, diluent buffer, species, antigen retrieval, incubation time, etc.
For the products you mentioned I can give a estimate for the pre-diluted antibodies of ca 140- 280slides, for the other antibodies 500 - 100 slides and for the detection kit 150 - 300 slides.
I hope this information is helpful.
Answered on Jan 26 2012