Recombinant
RabMAb

Recombinant Anti-Progesterone Receptor antibody [SP42] (ab101688)

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Overview

  • Product name

    Anti-Progesterone Receptor antibody [SP42]
    See all Progesterone Receptor primary antibodies

  • Description

    Rabbit monoclonal [SP42] to Progesterone Receptor

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Progesterone Receptor aa 400-550. The exact sequence is proprietary.
    Database link: P06401

  • Positive control

    • IHC-P: Human breast carcinoma, Human breast, Mouse breast, Rat breast FC: T-47D cells ICC/IF: T-47D cells

Properties

Applications

Our Abpromise guarantee covers the use of ab101688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/400. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate primary antibody for 30 minutes at room temperature.

Target

  • Function

    The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
    Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
    Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

  • Post-translational
    modifications

    Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
    Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
    Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.

  • Cellular localization

    Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
  • Information by UniProt

  • Database links

    • Alternative names

      • NR3C3 antibody
      • Nuclear receptor subfamily 3 group C member 3 antibody
      • PGR antibody
      • PR antibody
      • PRA antibody
      • PRB antibody
      • PRGR_HUMAN antibody
      • Progesterone receptor antibody
      • Progestin receptor form A antibody
      • Progestin receptor form B antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution (0.61 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on human breast cancer, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab101688 for 30 mins at room temperature.

    • Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP42] (ab101688)
      Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP42] (ab101688)
      Immunocytochemistry/ Immunofluorescence analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab101688 at 1:100 (2.66 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Flow Cytometry - Anti-Progesterone Receptor antibody [SP42] (ab101688)
      Flow Cytometry - Anti-Progesterone Receptor antibody [SP42] (ab101688)
      Flow Cytometry analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab101688 at 1:260 dilution (1.02 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat breast tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution (0.61 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on rat breast, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab101688 for 30 mins at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse breast tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution (0.61 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on mouse breast, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab101688 for 30 mins at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution (0.61 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Sporadically nuclear staining on human breast, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab101688 for 30 mins at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
      ab101688, at a 1/400 dilution, staining Progesterone Receptor in formalin fixed, paraffin embedded Human breast carcinoma by Immunohistochemistry.

    References

    ab101688 has not yet been referenced specifically in any publications.

    Publishing research using ab101688? Please let us know so that we can cite the reference in this datasheet.

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