Recombinant Anti-Progesterone Receptor antibody [SP42] (ab101688)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP42] to Progesterone Receptor
- Suitable for: Flow Cyt, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Progesterone Receptor antibody [SP42]
See all Progesterone Receptor primary antibodies -
Description
Rabbit monoclonal [SP42] to Progesterone Receptor -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human Progesterone Receptor aa 400-550. The exact sequence is proprietary.
Database link: P06401 -
Positive control
- IHC-P: Human breast carcinoma, Human breast, Mouse breast, Rat breastFlow Cyt: T-47D cells ICC/IF: T-47D cells
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP42 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
1/400. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate primary antibody for 30 minutes at room temperature.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
1/400. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate primary antibody for 30 minutes at room temperature. |
Target
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Function
The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Post-translational
modificationsPhosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation. -
Cellular localization
Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 5241 Human
- Entrez Gene: 18667 Mouse
- Entrez Gene: 25154 Rat
- Omim: 607311 Human
- SwissProt: P06401 Human
- SwissProt: Q00175 Mouse
- SwissProt: Q63449 Rat
- Unigene: 32405 Human
see all -
Alternative names
- NR3C3 antibody
- Nuclear receptor subfamily 3 group C member 3 antibody
- PGR antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution (0.61 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on human breast cancer, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101688 for 30 mins at room temperature. -
Immunocytochemistry/ Immunofluorescence analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab101688 at 1:100 (2.66 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Flow Cytometry analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab101688 at 1:260 dilution (1.02 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat breast tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution (0.61 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on rat breast, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101688 for 30 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse breast tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution (0.61 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on mouse breast, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101688 for 30 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue sections labeling Progesterone Receptor with ab101688 at 1/400 dilution (0.61 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Sporadically nuclear staining on human breast, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101688 for 30 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP42] (ab101688)ab101688, at a 1/400 dilution, staining Progesterone Receptor in formalin fixed, paraffin embedded Human breast carcinoma by Immunohistochemistry.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab101688 has not yet been referenced specifically in any publications.