Product nameAnti-Prohibitin antibody
See all Prohibitin primary antibodies
DescriptionRabbit polyclonal to Prohibitin
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Synthetic peptide within Human Prohibitin aa 222-272. The exact sequence is proprietary. NP_002625.1
Database link: P35232
- WB: HeLa and HEK-293T whole cell lysates. IHC-P: Human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 30 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionProhibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Tissue specificityWidely expressed in different tissues.
Sequence similaritiesBelongs to the prohibitin family.
Developmental stageLevels of expression in fibroblasts decrease heterogeneously during cellular aging.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
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All lanes : Anti-Prohibitin antibody (ab264264) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Exposure time: 3 minutes
Formalion-fixed, paraffin-embeded human breast carcinoma tissue stained for Prohibitin using ab264264 at 1/200 dilution in immunohistochemical analysis. DAB staining.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264264 has not yet been referenced specifically in any publications.