Recombinant
RabMAb

Recombinant Anti-Prohibitin antibody [EP2804Y] - BSA and Azide free (ab234919)

Overview

  • Product name

    Anti-Prohibitin antibody [EP2804Y] - BSA and Azide free
    See all Prohibitin primary antibodies
  • Description

    Rabbit monoclonal [EP2804Y] to Prohibitin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Prohibitin aa 150-250. The exact sequence is proprietary.

  • General notes

    Ab234919 is the carrier-free version of ab75771. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab234919 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.??

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab234919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 30 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. PubMed: 22125622
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
  • Tissue specificity

    Widely expressed in different tissues.
  • Sequence similarities

    Belongs to the prohibitin family.
  • Developmental stage

    Levels of expression in fibroblasts decrease heterogeneously during cellular aging.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Epididymis luminal protein 215 antibody
    • Epididymis secretory sperm binding protein Li 54e antibody
    • HEL 215 antibody
    • HEL S 54e antibody
    • PHB antibody
    • PHB_HUMAN antibody
    • PHB1 antibody
    • Prohibitin antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling Prohibitin with ab75711 at 1/250. An Alexa Fluor® 488-conjugated anti-rabbit IgG was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75771).

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Prohibitin with ab75711 at 1/250. An Alexa Fluor® 488-conjugated anti-rabbit IgG was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75771).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue labelling Prohibitin with ab75771 at 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75771).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling Prohibitin with ab75771 at 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75771).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse endometrium tissue labelling Prohibitin with ab75771 at 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75771).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Prohibitin with ab75771 at 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75771).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling Prohibitin with ab75771 at 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75771).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling Prohibitin with ab75771 at 1/100. A HRP/AP polymerized secondary antibody was used.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75771).

References

ab234919 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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