Recombinant
RabMAb

Recombinant Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771)

Overview

  • Product name
    Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker
    See all Prohibitin primary antibodies
  • Description
    Rabbit monoclonal [EP2804Y] to Prohibitin - Mitochondrial Marker
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Prohibitin aa 150-250. The exact sequence is proprietary.

  • Positive control
    • WB: MCF-7, PC-12, A431 and Ramos cell lysate; IHC-P: human hepatocellular carcinoma tissue, mouse kidney tissue, mouse endometrium tissue, mouse liver tissue, rat liver tissue and rat stomach tissue; ICC/IF: Hela and Neuro-2a cells. IP: MCF7
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab75771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 22125622
WB 1/500 - 1/1000. Predicted molecular weight: 30 kDa.
IP 1/20.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.

Target

  • Function
    Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
  • Tissue specificity
    Widely expressed in different tissues.
  • Sequence similarities
    Belongs to the prohibitin family.
  • Developmental stage
    Levels of expression in fibroblasts decrease heterogeneously during cellular aging.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis luminal protein 215 antibody
    • Epididymis secretory sperm binding protein Li 54e antibody
    • HEL 215 antibody
    • HEL S 54e antibody
    • PHB antibody
    • PHB_HUMAN antibody
    • PHB1 antibody
    • Prohibitin antibody
    see all

Images

  • All lanes : Anti-Prohibitin antibody [EP2804Y] - Mitochondrial Marker (ab75771) at 1/1000 dilution

    Lane 1 : MCF-7 cell lysates
    Lane 2 : PC-12 cell lysates
    Lane 3 : A431 cell lysates
    Lane 4 : Ramos cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Prohibitin with ab75771 at 1/250.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Prohibitin with ab75711 at 1/250. An Alexa Fluor® 488-conjugated anti-rabbit IgG was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling Prohibitin with ab75771 at 1/100. A HRP/AP polymerized secondary antibody was used.

  • ab75771 (purified) at 1/500 dilution (4.3 µg/ml) immunoprecipitating Prohibitin in MCF7 whole cell lysate .
    Lane 1 (input): MCF7(Human breast adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab75771 & MCF7 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75771 in MCF7 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM /TBST .

  • Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling Prohibitin with ab75711 at 1/250. An Alexa Fluor® 488-conjugated anti-rabbit IgG was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse endometrium tissue labelling Prohibitin with ab75771 at 1/250.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling Prohibitin with ab75771 at 1/250.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling Prohibitin with ab75771 at 1/250.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue labelling Prohibitin with ab75771 at 1/250.

References

This product has been referenced in:
  • Li Y  et al. Astragaloside IV reduces neuronal apoptosis and parthanatos in ischemic injury by preserving mitochondrial hexokinase-II. Free Radic Biol Med 131:251-263 (2019). Read more (PubMed: 30502455) »
  • Kosgodage US  et al. Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells. Transl Oncol 12:513-522 (2019). Read more (PubMed: 30597288) »
See all 9 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

The immunogen sequence is from between 150-200 amino acid of PHB Swissprot 35232. The immunogen sequence does not have any homology with PHB2, Swissprot Q99623.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

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Answer

Thank you very much for your patience.

I have hear back now from the laboratories in regards to the potential crossreaction of the prohibitin antibodies with T. brucei. Unfortunately none of these antibodies seems to be likely to crossreact with T. brucei. I am very sorry for this. Please find here the detailed comments:

ab115669: theexact sequence of the immunogen is proprietary. Upon alignment however with the T. brucei sequence, the homology is only 60%.
The immunogen of ab70672 it is located within aa 170-220.When comparing this immunogen sequence with the T.brucei sequence, the percentage homology is 47%.
Also for the ab75766, with an immunogen is N terminal, and ab75771, with an immunogen between in the middle, the alignment is not good enough to expect a crossreaction. Please see here the alignment of the human and T. brucei sequence http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20120213-172649-0982-54789458-pg

I am very sorry for not being able to provide you with better news. Please do let us know if there is anything else we could do to help you further.

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