Anti-Prohibitin antibody - Mitochondrial Marker (ab28172)
Key features and details
- Rabbit polyclonal to Prohibitin - Mitochondrial Marker
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Prohibitin antibody - Mitochondrial Marker
See all Prohibitin primary antibodies -
Description
Rabbit polyclonal to Prohibitin - Mitochondrial Marker -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P HumanWB MouseHuman -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Prohibitin.
-
Positive control
- Recombinant Human Prohibitin protein (ab114204) can be used as a positive control in WB. ab28172 antibody gave a positive signal in the following Whole Cell Lysates: HeLa, A431, Jurkat, NIH 3T3, MEF-1 and PC12. In addition, ab28172 antibody gave a positive signal in the following Tissue Lysates: Brain (Mouse), Liver (Mouse), Heart (Mouse), Kidney (Mouse), Pancreas (Mouse), Testis (Mouse), Skeletal Muscle (Mouse), Spinal Cord (Mouse), Ovary (Mouse), Brain (Rat), Liver (Rat) and Heart (Rat).
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC/IF |
Human
|
IHC-P |
Human
|
WB |
Mouse
Human
|
All applications |
Cow
Dog
Pig
|
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
|
|
ICC/IF | (2) |
Use a concentration of 5 µg/ml.
|
IHC-P | (3) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
Notes |
---|
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. -
Tissue specificity
Widely expressed in different tissues. -
Sequence similarities
Belongs to the prohibitin family. -
Developmental stage
Levels of expression in fibroblasts decrease heterogeneously during cellular aging. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
-
Database links
- Entrez Gene: 530409 Cow
- Entrez Gene: 480547 Dog
- Entrez Gene: 5245 Human
- Entrez Gene: 18673 Mouse
- Entrez Gene: 25344 Rat
- Omim: 176705 Human
- SwissProt: Q3T165 Cow
- SwissProt: P35232 Human
see all -
Alternative names
- Epididymis luminal protein 215 antibody
- Epididymis secretory sperm binding protein Li 54e antibody
- HEL 215 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody - Mitochondrial Marker (ab28172)IHC image of Prohibitin staining in human liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28172, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
-
All lanes : Anti-Prohibitin antibody - Mitochondrial Marker (ab28172) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 5 : Heart (Mouse) Tissue Lysate
Lane 6 : Kidney (Mouse) Tissue Lysate
Lane 7 :Mouse pancreas tissue lysate - total protein (ab29363)
Lane 8 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 9 :Mouse skeletal muscle tissue lysate - total protein (ab29711)
Lane 10 : Spinal Cord (Mouse) Tissue Lysate
Lane 11 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 12 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 13 : Brain (Rat) Tissue Lysate - normal tissue
Lane 14 : Liver (Rat) Tissue Lysate
Lane 15 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa -
ab28172 staining Prohibitin in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton for 1h. The cells were then incubated with the antibody ab28172 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody - Mitochondrial Marker (ab28172)This image is courtesy of an anonymous Abreview
ab28172 staining Prohibitin in mouse liver tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking in 1.5% serum for 10 minutes. The primary antibody was diluted, 1/400 and incubated with sample for 1 hour. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
-
All lanes : Anti-Prohibitin antibody - Mitochondrial Marker (ab28172) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa -
ICC/IF image of ab28172 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab28172, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence. 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (48)
ab28172 has been referenced in 48 publications.
- Trentzsch M et al. Delivery of phosphatidylethanolamine blunts stress in hepatoma cells exposed to elevated palmitate by targeting the endoplasmic reticulum. Cell Death Discov 6:8 (2020). PubMed: 32123584
- Hensen F et al. Mitochondrial RNA granules are critically dependent on mtDNA replication factors Twinkle and mtSSB. Nucleic Acids Res N/A:N/A (2019). PubMed: 30715486
- Huang C et al. FoxM1 Induced Paclitaxel Resistance via Activation of the FoxM1/PHB1/RAF-MEK-ERK Pathway and Enhancement of the ABCA2 Transporter. Mol Ther Oncolytics 14:196-212 (2019). PubMed: 31334335
- Sugiyama Y et al. Neuronal and microglial localization of secreted phosphoprotein 1 (osteopontin) in intact and damaged motor cortex of macaques. Brain Res 1714:52-64 (2019). PubMed: 30790559
- Liu C et al. Testosterone Deficiency Caused by Castration Modulates Mitochondrial Biogenesis Through the AR/PGC1a/TFAM Pathway. Front Genet 10:505 (2019). PubMed: 31191617