Anti-Prohibitin antibody - Mitochondrial Marker (ab28172)

Thank you for your phone call.

I sincerely apologize for the incorrect advice that was given to you originally.

Out of courtesy and to correct our mistake, I have issued a free of charge replacement with the order number xxx with ab55618, which is better suited for conjugation. You should receive the antibody tomorrow.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you very much for your patience.

I have hear back now from the laboratories in regards to the potential crossreaction of the prohibitin antibodies with T. brucei. Unfortunately none of these antibodies seems to be likely to crossreact with T. brucei. I am very sorry for this. Please find here the detailed comments:

ab115669: theexact sequence of the immunogen is proprietary. Upon alignment however with the T. brucei sequence, the homology is only 60%.
The immunogen of ab70672 it is located within aa 170-220.When comparing this immunogen sequence with the T.brucei sequence, the percentage homology is 47%.
Also for the ab75766, with an immunogen is N terminal, and ab75771, with an immunogen between in the middle, the alignment is not good enough to expect a crossreaction. Please see here the alignment of the human and T. brucei sequence http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20120213-172649-0982-54789458-pg

I am very sorry for not being able to provide you with better news. Please do let us know if there is anything else we could do to help you further.

Thank you very much for your interest in our antibodies.

We unfortunately do not have small size aliquots of our products, however we can offer you a testing discount on an antibody to test it in a new species. Please see below for further details.

In regards to the choice of prohibitin antibody, I would not recommend to test the ab28172. Indeed, I performed the alignment of the immunogen with the T. brucei sequence, and there is only 50% identity.

I have requested immunogen information for the other antibodies against prohibitin, in order to provide an alignment and to predict crossreactivity. I will get back to you as soon as I have more information. Many of our anti prohibitin antibodies are however monoclonal antibodies generated against the whole protein and wedonot know the epitope of these (ab79198, ab1836, ab59773 and ab54330).

If you would liketotest then one of these antibodies, I can offer a discount off a future purchase if you buythat antibodynow, test it inT.bruceiand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

0.) Wait until I get back to you with some more information on the probable crossreaction of our other antibodies with the T. brucei prohibitin

1. Reply to this e-mail to let me know that you would like to proceed and test the antibody in T. brucei. I will then send a discount code. This code must be issued before purchasingthe antibodyso please wait for my reply before ordering.

2. Purchase the antibody either by phone, fax, or online (www.abcam.com).

3. Test it in T. brucei.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe antibodyturns out to be unsuitable for T. brucei, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer or in regards to the predicted crossreactivity of the antibodies, and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.