Overview

  • Product name

    Anti-Prohibitin antibody [PHB/1881]
    See all Prohibitin primary antibodies
  • Description

    Mouse monoclonal [PHB/1881] to Prohibitin
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Prohibitin aa 167-261. The exact sequence is proprietary.
    Database link: P35232-1

  • Positive control

    • IHC-P: Human liver tissue. WB: HEK-293, Jurkat, HepG2 and U-87 MG cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab224653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 30 kDa.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
  • Tissue specificity

    Widely expressed in different tissues.
  • Sequence similarities

    Belongs to the prohibitin family.
  • Developmental stage

    Levels of expression in fibroblasts decrease heterogeneously during cellular aging.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Epididymis luminal protein 215 antibody
    • Epididymis secretory sperm binding protein Li 54e antibody
    • HEL 215 antibody
    • HEL S 54e antibody
    • PHB antibody
    • PHB_HUMAN antibody
    • PHB1 antibody
    • Prohibitin antibody
    see all

Images

  • All lanes : Anti-Prohibitin antibody [PHB/1881] (ab224653) at 1 µg/ml

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 4 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate

    Developed using the ECL technique.

    Predicted band size: 30 kDa

  • Formalin-fixed, paraffin-embedded human liver tissue stained for Prohibitin with ab224653 at 2 µg/ml in immunohistochemical analysis.

References

ab224653 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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