Key features and details
- Mouse monoclonal [PHB/1882] to Prohibitin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Prohibitin antibody [PHB/1882]
See all Prohibitin primary antibodies
DescriptionMouse monoclonal [PHB/1882] to Prohibitin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Prohibitin aa 167-261. The exact sequence is proprietary.
Database link: P35232
- IHC-P: Human liver tissue. WB: A549, K562, A431 and MCF7 cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from bioreactor concentrate.
Light chain typekappa
Our Abpromise guarantee covers the use of ab224657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 30 kDa.|
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionProhibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Tissue specificityWidely expressed in different tissues.
Sequence similaritiesBelongs to the prohibitin family.
Developmental stageLevels of expression in fibroblasts decrease heterogeneously during cellular aging.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- Epididymis luminal protein 215 antibody
- Epididymis secretory sperm binding protein Li 54e antibody
- HEL 215 antibody
All lanes : Anti-Prohibitin antibody [PHB/1882] (ab224657) at 1 µg/ml
Lane 1 : A549 (human lung carcinoma cell line) cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 30 kDa
Formalin-fixed, paraffin-embedded human liver tissue stained for Prohibitin with ab224657 at 2 µg/ml in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224657 has not yet been referenced specifically in any publications.