Anti-Prokineticin 2/PK2 antibody (ab76747)

Submit a review Q&A (6)References (1)

Overview

  • Product name

    Anti-Prokineticin 2/PK2 antibody
    See all Prokineticin 2/PK2 primary antibodies

  • Description

    Rabbit polyclonal to Prokineticin 2/PK2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human Prokineticin 2/PK2 (C terminal).
    (Peptide available as ab140536)

  • General notes

     This product was previously labelled as Prokineticin 2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab76747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Predicted molecular weight: 14 kDa.
IHC-Fr 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.

Target

  • Function

    May function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. May also function locally within the SCN to synchronize output. Potently contracts gastrointestinal (GI) smooth muscle.

  • Tissue specificity

    Expressed in the testis and, at low levels, in the small intestine.

  • Involvement in disease

    Defects in PROK2 are the cause of Kallmann syndrome type 4 (KAL4) [MIM:610628]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. KAL4 patients have variable degrees of olfactory and reproductive dysfunction, but do not show any of the occasional clinical anomalies reported in Kallmann syndrome such as renal agenesis, cleft lip/palate, selective tooth agenesis, and bimanual synkinesis.

  • Sequence similarities

    Belongs to the AVIT (prokineticin) family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • BV8 antibody
    • Bv8 homolog antibody
    • MIT1 antibody
    • PK2 antibody
    • PROK2 antibody
    • PROK2_HUMAN antibody
    • Prokineticin-2 antibody
    • Protein Bv8 homolog antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prokineticin 2/PK2 antibody (ab76747)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prokineticin 2/PK2 antibody (ab76747)

    A: ab76747 staining Prokinectin 2 in Mouse testis by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    B: Negative control treated with preimmune serum.
    Both sections counterstained with haematoxylin.

References

This product has been referenced in:

  • Mundim MV  et al. A new function for Prokineticin 2: Recruitment of SVZ-derived neuroblasts to the injured cortex in a mouse model of traumatic brain injury. Mol Cell Neurosci 94:1-10 (2019). Read more (PubMed: 30391355) »
See 1 Publication for this product

Publishing research using ab76747? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-6 of 6 Q&A

Question

I want to know whether ab76747 cross reacts with rat.

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Answer

Yes, the antibody does cross-react with rat PK2, and also mouse.

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Question

Will this antibody react with mouse protein?

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Answer

Yes ab76747 cross reacts with mouse.

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Question

Thank you for your quick answer.

Can I have an idea of he discount you will give for our results?

Plus, you didn't answer for the fixation protocole if we test your antibody in a IF assay.. (or another antibody actually), can we use PFA without risk?

Thanks a lot

Best Regards

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Answer

Thank you for your response.

As in Point 2 in the previous e-mail describes, the discount code will be for the original value of the product. You can use and test different fixative to chec which one would work better in your experimental system such as 4% PFA (rrom temperature), methanol, acetone, ethnaol (ice-cold).


If you need any further assistance in the future, please do not hesitate to contact me.

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Question

Hello, I have a question regarding your antibody ab76747
You explained that it works in human and it is predicted to work in both rats and mice.
We are working on those animal models, could we try to use a sample of your antibody in either WB or IHC for free and give you the result to assess whether or not it works in rodents?

Plus, if we want to use IF with your antibodies, do you recommand a fixation in PFA or not?

Thanks a lot for your time and cooperation

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Answer

Thank you very much for your interest our AbTrial program.


To our knowledge, ab76747 has not been tested in murine system. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

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Question

Good afternoon,



I am Ph.D. student at University of Rome ‘La Sapienza’.

I want to know if do you have the Bloking peptide against the Anti-Prokineticin 2 antibody (ab76747)?



Best wishes,

Read More
Answer

Thank you for your inquiry.


I am happy to confirm that we now have added the blocking peptide for this antibody to out catalog.

Please follow this link to the datasheet of the blocking peptide:

ab140536

https://www.abcam.com/index.html?datasheet=140536 (or use the following: https://www.abcam.com/index.html?datasheet=140536).

I hope this information is helpful and wish you good luck with your research.

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Question

Sir/Madam, We are interested in the anti-Prokineticin 2 antibody, but have some questions: 1. Have you validation of this as a radioimmunoassay? 2. Do have any sensitivity, specificity, cross-reactant data?

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Answer

Thank you very much for your interest in ab76747.
To our knowledge, ab76747 has not been tested in radioimmunoassay (RIA) and we predict that mouse and rat tissue. Therefore, I can offer a discount off a future purchase if you buy ab76747 now, test it in RIA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed and test ab76747 in RIA. I will then send a discount code. This code must be issued before purchasing ab76747 so please wait for my reply before ordering.
2. Purchase ab76747 either by phone, fax, or online (www.abcam.com).
3. Test it in RIA.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab76747 turns out to be unsuitable for RIA, you will still receive the discount on your next purchase after your Abreview has been submitted.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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