Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab119999 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab119999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 34 kDa. Preliminary experiments gave an approx 45kDa band in Mouse Eye lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.6kDa according to NP_032670.2. The 45kDa band was successfully blocked by incubation with the immunizing peptide.|
|ICC/IF||Use a concentration of 3 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
RelevanceOpiorphin is an endogenous inhibitor of neprilysin and aminopeptidase N. Inhibits the breakdown of substance P, Mca-BK2 and Met-enkephalin by neprilysin in vitro with IC50 values of 29 µM, 33 µM and 33 µM respectively. Inhibits the breakdown of Ala-pNA by aminopeptidase N in vitro with an IC50 of 65 µM. Has a potent analgesic effect when administered to rats by intravenous injection.
- Entrez Gene: 17830 Mouse
- Basic proline rich lacrimal protein antibody
- Basic proline rich protein antibody
- BPLP antibody