Recombinant
RabMAb

Anti-Prolactin Receptor antibody [EPR7184(2)] (ab170935)

Overview

  • Product name
    Anti-Prolactin Receptor antibody [EPR7184(2)]
    See all Prolactin Receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR7184(2)] to Prolactin Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Prolactin Receptor aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P16471

  • Positive control
    • T47-D, (B) A431 and (C) Jurkat cell lysates, Human breast carcinoma & kidney tissue, Jurkat cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 70 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ab170935 shows background nuclear staining on mouse colon,pancreas and lung.

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
    • Tissue specificity
      Expressed in breast, placenta, kidney, liver and pancreas.
    • Sequence similarities
      Belongs to the type I cytokine receptor family. Type 1 subfamily.
      Contains 2 fibronectin type-III domains.
    • Domain
      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is required for JAK interaction and/or activation.
    • Cellular localization
      Secreted and Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI987712 antibody
      • CLONE SPM213 antibody
      • CPRLP antibody
      • Delta 4-delta 7/11 truncated prolactin receptor antibody
      • Delta 4-SF1b truncated prolactin receptor antibody
      • HPRL antibody
      • hPRL receptor antibody
      • hPRLrI antibody
      • Lactogen receptor antibody
      • MFAB antibody
      • MGC105486 antibody
      • OPR antibody
      • OTTHUMP00000115998 antibody
      • Pr-1 antibody
      • Pr-3 antibody
      • PRL R antibody
      • PRL-R antibody
      • PRLR antibody
      • Prlr-rs1 antibody
      • PRLR_HUMAN antibody
      • Prolactin receptor a antibody
      • Prolactin receptor antibody
      • Prolactin receptor delta 7/11 antibody
      • RATPRLR antibody
      • Secreted prolactin binding protein antibody
      • Truncated testis-specific box 1-C prolactin receptor antibody
      • wu:fj65c07 antibody
      see all

    Images

    • All lanes : Anti-Prolactin Receptor antibody [EPR7184(2)] (ab170935) at 1/1000 dilution

      Lane 1 : T47-D cell lysates
      Lane 2 : A431 cell lysates
      Lane 3 : Jurkat cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 70 kDa

    • Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue labeling Prolactin Receptor with ab170935 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded kidney tissue labeling Prolactin Receptor with ab170935 at 1/50 dilution.

    • Immunofluorescence analysis of Jurkat cells labeling Prolactin Receptor with ab170935 at 1/100 dilution.

    References

    This product has been referenced in:
    • Gao Z  et al. Expression of Stem Cell Markers and Dopamine D2 Receptors in Human and Rat Prolactinomas. Med Sci Monit 23:1827-1833 (2017). IHC . Read more (PubMed: 28411401) »
    • Yu L  et al. MFGE8 regulates TGF-ß-induced epithelial mesenchymal transition in endometrial epithelial cells in vitro. Reproduction 152:225-33 (2016). ICC/IF ; Human . Read more (PubMed: 27340235) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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