Product nameProlactin Receptor overexpression 293T lysate (whole cell)
ab94257 is a 293T cell transfected lysate in which Human Prolactin Receptor / PRL-R has been transiently over-expressed using a pCMV-Prolactin Receptor plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling. Tissue specificity: Expressed in breast, placenta, kidney, liver and pancreas. Similarity: Belongs to the type I cytokine receptor family. Type 1 subfamily. Contains 2 fibronectin type-III domains. Domain: The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
Our Abpromise guarantee covers the use of ab94257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94257 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-Prolactin Receptor/PRL-R antibody (ab89215) at 1/500 dilution
Lane 1 :
Prolactin Receptor overexpression 293T lysate (whole cell) (ab94257)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94257 has not yet been referenced specifically in any publications.