Anti-Prolactin Receptor/PRL-R antibody [B6.2 + PRLR742] (ab199015)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Prolactin Receptor/PRL-R antibody [B6.2 + PRLR742]
See all Prolactin Receptor/PRL-R primary antibodies -
Description
Mouse monoclonal [B6.2 + PRLR742] to Prolactin Receptor/PRL-R -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, IHC-Fr, ICC/IF, IP, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
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Positive control
- IHC-P: Human testicular carcinoma tissue.
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General notes
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Previously labelled as Prolactin Receptor.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab199015 was purified from Bioreactor Concentrate. -
Clonality
Monoclonal -
Clone number
B6.2 + PRLR742 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab199015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 30 minutes at RT. |
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| WB | Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 70 kDa. | |
| IHC-Fr | Use a concentration of 0.5 - 1 µg/ml. 30 minutes at RT. |
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| ICC/IF | Use a concentration of 0.5 - 1 µg/ml. | |
| IP | Use at 1-2 µg/mg of lysate. | |
| Flow Cyt | Use 0.5-1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling. -
Tissue specificity
Expressed in breast, placenta, kidney, liver and pancreas. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 1 subfamily.
Contains 2 fibronectin type-III domains. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Secreted and Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5618 Human
- Omim: 176761 Human
- SwissProt: P16471 Human
- Unigene: 368587 Human
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Alternative names
- AI987712 antibody
- CLONE SPM213 antibody
- CPRLP antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [B6.2 + PRLR742] (ab199015)](https://www.abcam.com/ps/products/199/ab199015/Images/ab199015-369036-anti-prolactin-receptor-antibody-immunohistochemistry-testicular-carcinoma-tissue.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [B6.2 + PRLR742] (ab199015)Formalin-fixed, paraffin-embedded human Testicular Carcinoma tissue stained for Prolactin Receptor/PRL-R using ab199015 at 2 µg/ml in immunohistochemical analysis.
Protocols
Datasheets and documents
References
ab199015 has not yet been referenced specifically in any publications.
