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  1. Link

    prolactin-receptorprl-r-antibody-b62--prlr742-ab199015.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases
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Anti-Prolactin Receptor/PRL-R antibody [B6.2 + PRLR742] (ab199015)

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  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [B6.2 + PRLR742] (ab199015)

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Primary

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Secondary

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Protein

 

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-Prolactin Receptor/PRL-R antibody [B6.2 + PRLR742]
    See all Prolactin Receptor/PRL-R primary antibodies
  • Description

    Mouse monoclonal [B6.2 + PRLR742] to Prolactin Receptor/PRL-R
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, IHC-Fr, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    This product was produced with the following immunogens:
    Recombinant full length protein corresponding to Human Prolactin Receptor/PRL-R. (PRLR742)
    Database link: P16471

    Recombinant full length protein corresponding to Human Prolactin Receptor/ PRL-R. Semi-purified. (B6.2)
    Database link: P16471

  • Positive control

    • IHC-P: Human testicular carcinoma tissue.
  • General notes

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

    Previously labelled as Prolactin Receptor. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab199015 was purified from Bioreactor Concentrate.
  • Clonality

    Monoclonal
  • Clone number

    B6.2 + PRLR742
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Signal Transduction
    • Metabolism
    • Lipid metabolism

Associated products

  • Alternative Versions

    • Anti-Prolactin Receptor/PRL-R antibody [B6.2 + PRLR742] - BSA and Azide free (ab269737)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant rabbit Prolactin Receptor/PRL-R protein (ab73248)

Applications

Our Abpromise guarantee covers the use of ab199015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

30 minutes at RT.

WB Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 70 kDa.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.

30 minutes at RT.

ICC/IF Use a concentration of 0.5 - 1 µg/ml.
IP Use at 1-2 µg/mg of lysate.
Flow Cyt Use 0.5-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
  • Tissue specificity

    Expressed in breast, placenta, kidney, liver and pancreas.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 1 subfamily.
    Contains 2 fibronectin type-III domains.
  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localization

    Secreted and Membrane.
  • Target information above from: UniProt accession P16471 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5618 Human
    • Omim: 176761 Human
    • SwissProt: P16471 Human
    • Unigene: 368587 Human
    • Alternative names

      • AI987712 antibody
      • CLONE SPM213 antibody
      • CPRLP antibody
      • Delta 4-delta 7/11 truncated prolactin receptor antibody
      • Delta 4-SF1b truncated prolactin receptor antibody
      • HPRL antibody
      • hPRL receptor antibody
      • hPRLrI antibody
      • Lactogen receptor antibody
      • MFAB antibody
      • MGC105486 antibody
      • OPR antibody
      • OTTHUMP00000115998 antibody
      • Pr-1 antibody
      • Pr-3 antibody
      • PRL R antibody
      • PRL-R antibody
      • PRLR antibody
      • Prlr-rs1 antibody
      • PRLR_HUMAN antibody
      • Prolactin receptor a antibody
      • Prolactin receptor antibody
      • Prolactin receptor delta 7/11 antibody
      • RATPRLR antibody
      • Secreted prolactin binding protein antibody
      • Truncated testis-specific box 1-C prolactin receptor antibody
      • wu:fj65c07 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [B6.2 + PRLR742] (ab199015)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [B6.2 + PRLR742] (ab199015)

      Formalin-fixed, paraffin-embedded human Testicular Carcinoma tissue stained for Prolactin Receptor/PRL-R using ab199015 at 2 µg/ml in immunohistochemical analysis.

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Datasheets and documents

    • Datasheet
  • References

    ab199015 has not yet been referenced specifically in any publications.

    Publishing research using ab199015? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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