RecombinantRabMAb

Recombinant Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935)

Reviews (1) Submit a question References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935)
  • Western blot - Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935)
  • Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935)
  • Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7184(2)] to Prolactin Receptor/PRL-R
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)]
    See all Prolactin Receptor/PRL-R primary antibodies

  • Description

    Rabbit monoclonal [EPR7184(2)] to Prolactin Receptor/PRL-R

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Prolactin Receptor/PRL-R aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P16471

  • Positive control

    • T47-D, (B) A431 and (C) Jurkat whole cell lysate (ab7899), Human breast carcinoma & kidney tissue, Jurkat cells

  • General notes

    Previously labelled as Prolactin Receptor. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab170935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 70 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ab170935 shows background nuclear staining on mouse colon,pancreas and lung.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.

    • Tissue specificity

      Expressed in breast, placenta, kidney, liver and pancreas.

    • Sequence similarities

      Belongs to the type I cytokine receptor family. Type 1 subfamily.
      Contains 2 fibronectin type-III domains.

    • Domain

      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is required for JAK interaction and/or activation.

    • Cellular localization

      Secreted and Membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • AI987712 antibody
      • CLONE SPM213 antibody
      • CPRLP antibody
      • Delta 4-delta 7/11 truncated prolactin receptor antibody
      • Delta 4-SF1b truncated prolactin receptor antibody
      • HPRL antibody
      • hPRL receptor antibody
      • hPRLrI antibody
      • Lactogen receptor antibody
      • MFAB antibody
      • MGC105486 antibody
      • OPR antibody
      • OTTHUMP00000115998 antibody
      • Pr-1 antibody
      • Pr-3 antibody
      • PRL R antibody
      • PRL-R antibody
      • PRLR antibody
      • Prlr-rs1 antibody
      • PRLR_HUMAN antibody
      • Prolactin receptor a antibody
      • Prolactin receptor antibody
      • Prolactin receptor delta 7/11 antibody
      • RATPRLR antibody
      • Secreted prolactin binding protein antibody
      • Truncated testis-specific box 1-C prolactin receptor antibody
      • wu:fj65c07 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935)

      Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Prolactin Receptor / PRL-R with ab170935 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935)
      Western blot - Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935)
      All lanes : Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935) at 1/1000 dilution

      Lane 1 : T47-D cell lysates
      Lane 2 : A431 cell lysates
      Lane 3 : Jurkat cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 70 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Prolactin Receptor / PRL-R with ab170935 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935)
      Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935)

      Immunofluorescence analysis of Jurkat cells labeling Prolactin Receptor / PRL-R with ab170935 at 1/100 dilution.

    • Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935)
      Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)] (ab170935)

    References (3)

    Publishing research using ab170935? Please let us know so that we can cite the reference in this datasheet.

    ab170935 has been referenced in 3 publications.

    • Zhang P  et al. Prolactin improves hepatic steatosis via CD36 pathway. J Hepatol 68:1247-1255 (2018). IHC-P ; Human . PubMed: 29452209
    • Gao Z  et al. Expression of Stem Cell Markers and Dopamine D2 Receptors in Human and Rat Prolactinomas. Med Sci Monit 23:1827-1833 (2017). IHC . PubMed: 28411401
    • Yu L  et al. MFGE8 regulates TGF-ß-induced epithelial mesenchymal transition in endometrial epithelial cells in vitro. Reproduction 152:225-33 (2016). ICC/IF ; Human . PubMed: 27340235

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Prolactin Receptor/PRL-R antibody [EPR7184(2)]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Mammary gland of nursing mouse)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ctitrate buffer pH6
    Permeabilization
    No
    Specification
    Mammary gland of nursing mouse
    Blocking step
    5% BSA 3% horse serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Flavia Ghiraldini

    Verified customer

    Submitted Sep 26 2018

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