Recombinant
RabMAb

Recombinant Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free (ab226491)

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Overview

  • Product name

    Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free
    See all Prolactin/PRL primary antibodies

  • Description

    Rabbit monoclonal [EPR18018-31] to Prolactin/PRL - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Mouse Prolactin/PRL aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P06879

  • Positive control

    • IHC-P: Mouse pituitary tissue.

  • General notes

    Ab226491 is the carrier-free version of ab188229. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab226491 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18018-31

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 23 kDa (predicted molecular weight: 25 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This antibody is not suitable for human species in IHC application.
IHC-Fr Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with 10 mM sodium citrate buffer pH 6.0 + 0.05% Tween-20 before commencing with IHC staining protocol.

Under our IHC-Fr experimental conditions this antibody could not detect Prolactin on rat pituitary gland sample.

 
IP Use at an assay dependent concentration.

Target

Images

  • Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free (ab226491)
    Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free (ab226491)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse pituitary tissue labeling Prolactin/PRL with ab188229 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining in lactotrophic cells of anterior pituitary gland (PMID: 16468032). The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188229).

  • Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free (ab226491)
    Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free (ab226491)

    Prolactin/PRL was immunoprecipitated from 0.35 mg of mouse pituitary tissue lysate with ab188229 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188229 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse pituitary tissue lysate 10 µg (Input). 

    Lane 2: ab188229 IP in mouse pituitary tissue lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188229 in mouse pituitary tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188229).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free (ab226491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free (ab226491)

    Immunohistochemical analysis of paraffin-embedded rat pituitary tissue labeling Prolactin/PRL with ab188229 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat pituitary (PMID: 24514953). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188229).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free (ab226491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin/PRL antibody [EPR18018-31] - BSA and Azide free (ab226491)

    Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue labeling Prolactin/PRL with ab188229 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse pituitary (PMID: 24514953). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188299).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab226491 has not yet been referenced specifically in any publications.

Publishing research using ab226491? Please let us know so that we can cite the reference in this datasheet.

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